中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 混菌固态发酵制备玉米皮菌体蛋白饲料的培养基优化 中国饲料 2013 (10) 16-19
Position: Home > Articles > Optimization of culture medium for production of microbial biomass protein from corn bran by two mixed strain using solid-state fermentation China Feed 2013 (10) 16-19

混菌固态发酵制备玉米皮菌体蛋白饲料的培养基优化

作  者：
王伟伟;齐景伟;安晓萍;罗旭光;乌兰;陈大勇;仝宝生
单  位：
内蒙古农业大学动物科学学院;内蒙古斯隆生物技术有限责任公司
关键词：
Plackett-Burman;响应面分析法;玉米皮;真蛋白质
摘  要：
选择Plackett-Burman设计和响应面优化相结合的方法,对米曲霉CGMCC 2.0951和热带假丝酵母CGMCC 2.118混合固态发酵玉米皮制备菌体蛋白质饲料的培养基组成进行了优化。二水平PB设计对影响玉米皮发酵的8个因素进行筛选,得出影响最大的3个因素:麸皮、尿素和K2HPO4;再通过响应面分析法对这3个因素进行优化,得出最适添加量分别为麸皮7.63%、尿素1.54%和K2HPO41.16%。优化后的玉米皮培养基经过混菌发酵,其真蛋白质含量可达到12.54%,较发酵原料提高了1.65倍。
译  名：
Optimization of culture medium for production of microbial biomass protein from corn bran by two mixed strain using solid-state fermentation
作  者：
Wang Weiwei,Qi Jingwei,An Xiaoping,et al
关键词：
Plackett-Burman design;response surface methodology;corn bran;true protein
摘  要：
Two stage statistical design was used to optimize microbial biomass protein production from Aspergillus oryzae CGMCC2.0951 and Candida tropicalis CGMCC2.118 under solid-state fermentation.Initially,Plackett–Burman designing(PB) was used for the selection of crucial production parameters.Wheat bran、urea and K2HPO4 were found to be the crucial factors for the microbial biomass protein production.Crucial variables were further processed through Box Behnken of response surface methodology(RSM) to maximize the true protein yield.Three factors were investigated at three different levels to study their influence on true protein production.Statistical approach showed that the optimum conditions for true protein production(wheat bran 7.63%,urea 1.54%and K2HPO4 1.16%) resulted in 1.65-fold increase in true protein yield(12.54%) over raw materials.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46