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Position: Home > Articles > Slecting the Most Suitable Conditions for Preparing Iron-adapted Yeast Journal of Sichuan Agricultural University 2010,28 (1) 55-58

啤酒酵母富铁培养条件筛选

作  者:
许祯莹;陈代文;余冰
单  位:
四川农业大学动物营养研究所
关键词:
富铁酵母;富集参数;驯化
摘  要:
本实验研究啤酒酵母菌生长特性以及不同pH和培养基起始铁浓度对酵母菌生长的影响,驯化菌株的耐铁能力,筛选培养基的最佳起始铁浓度,确定富铁酵母生产的适宜参数。结果表明:啤酒酵母菌适应起始pH值为5~7,偏酸性;培养时间为48 h;确定培养基铁浓度为800 mg/L时开始驯化菌株以制备耐铁酵母菌。利用驯化后的酵母菌,在起始pH值为6.0,铁添加量为800 mg/L的条件下,150 r/min,30℃振荡培养48 h,酵母菌的生长量为12.9 g/L,菌体铁的含量可达23.94 mg/g风干菌体,有机化程度为98.14%。
译  名:
Slecting the Most Suitable Conditions for Preparing Iron-adapted Yeast
作  者:
XU Zhen-ying,CHEN Dai-wen,YU Bing(Animal Nutrition Institute,Sichuan Agricultural University,Yaan 625014,Sichuan,China)
关键词:
iron-enriched yeast;the parameter of fermentation;naturalization
摘  要:
Exp.aims to seek for more suitable conditions for preparing iron-adapted yeast by investigating the character,the initial pH and iron concentration in medium of Saccharomyces cerevisia.The results suggested that Saccharomyces cerevisia can adapt and grow well between the pH value 5~7(nature pH) with 800 mg/L iron condition for 48 h.We chose these iron concentration as the initial concentration of naturalization.In the last,yeast was cultured in natural medium with initial pH 6.0,iron concentration 800 mg/L for 48 h.The yeast output was 12.9 g/L and contained iron 23.94 mg/g with 98.14% of iron being organic.

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