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Position: Home > Articles > Preparation of Polyclonal Antibody against Bovine Resistin and the Establishment of ELISA Method China Animal Husbandry & Veterinary Medicine 2009,36 (11) 84-87

牛抵抗素多克隆抗体的制备及ELISA检测方法的建立

作  者:
孙佳;李小兵;孔涛;许超;王照鹏;杨威;刘磊;王哲
单  位:
吉林大学畜牧兽医学院
关键词:
抵抗素;重组蛋白;ELISA
摘  要:
用纯化的可溶性重组牛抵抗素蛋白免疫健康家兔,制备了多克隆抗体,并对多克隆抗体进行了纯化、效价测定、特异性检测,初步建立了检测牛抵抗素的间接ELISA方法。
译  名:
Preparation of Polyclonal Antibody against Bovine Resistin and the Establishment of ELISA Method
作  者:
SUN Jia,LI Xiao-bing,KONG Tao,XU Chao,WANG Zhao-peng,YANG Wei,LIU Lei,WANG Zhe(College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China)
关键词:
resistin;recombinant protein;ELISA
摘  要:
The purified solubility recombinant resistin was used as antigen for immunizing rabbit to obtain resistin polyclonal antibody.The polyclonal antibody was purified,titer and specificity was determined.Then indirect ELISA action for cattle resistin detection was established.

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