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Position: Home > Articles > Determination of Tulathromycinin in Injection by HPLC-ELSD Method China Animal Husbandry & Veterinary Medicine 2012,39 (7) 256-259

HPLC-ELSD测定注射液中泰拉霉素的含量

作  者:
范炜达;陈良柱;贺利民;方炳虎
单  位:
华南农业大学兽医学院;广东大华农动物保健品股份有限公司
关键词:
泰拉霉素;注射液;高效液相色谱-蒸发光散射检测法;含量测定
摘  要:
本研究建立了注射液中泰拉霉素的高效液相色谱-蒸发光散射检测法。试样为自制5%泰拉霉素注射液,以ODSC18(250μm×4.6μm,5μm)为色谱柱,2 mmol/L乙酸铵水溶液(含0.1%甲酸)-乙腈(80∶20)为流动相,流速为1mL/min,柱温25℃,蒸发光散射检测器条件:漂移管温度60℃,喷嘴加热器级别为60%,气体压力为172.4kpa,增益为150。研究结果表明,泰拉霉素在0.01~0.2mg/mL范围内,呈良好的线性关系(R=0.9989),在高、中、低3个加标水平下的平均回收率为96.3%~97.8%,日间变异系数为0.6%~0.9%。该方法简便、快捷、准确,可用于泰拉霉素注射液的含量检测。
译  名:
Determination of Tulathromycinin in Injection by HPLC-ELSD Method
作  者:
FAN Wei-da1,CHEN Liang-zhu2,HE Li-min1,FANG Bing-hu1,2(1.Department of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;2.Guangdong Dahuanong Animal Health Products Co.,Ltd.,Xinxing 527400,China)
关键词:
tulathromycin;injection;HPLC-ELSD;content determination
摘  要:
A high performance liquid chromatograph-evaporative light-scattering detector(HPLC-ELSD) was developed for the content determination of tulathromycin in injection.The sample was injectable formulation containing 50 mg/mL tulathromycin.The chromatographic condition were ODS C18(250 μm×4.6μm,5 μm).The mobile phase was 0.02 mol/L ammonium acetate with formic acid-acetonitrile(80∶20),flow rate was 1 mL/min;column temperature was 25 ℃;evaporative light-scattering detector(ELSD): drift tube temperature was 60 ℃;nebulizer heater level was 60%;gas pressure was 172.4 kpa,PMT gain was 150.The calibration curve was linear in the range of 0.01 to 0.2 mg/mL with correlation coefficient 0.9989,the spiked recoveries at three spiked concentration levels were in the range of 96.3% to 97.8%,the RSDs were 0.6% to 0.9%.The results showed that this method was simple,quick and accurate for the content determination of tulathromycin in injection.

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