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当前位置: 首页 > 文章 > 离体筛选彩叶凤梨耐羟脯氨酸变异体的研究 山西农业科学 2018 (9) 1455-1457,1487
Position: Home > Articles > 离体筛选彩叶凤梨耐羟脯氨酸变异体的研究 Journal of Shanxi Agricultural Sciences 2018 (9) 1455-1457,1487

离体筛选彩叶凤梨耐羟脯氨酸变异体的研究

作  者：
崔克强;王彦尊;史华平;王计平
关键词：
EMS;彩叶凤梨;诱变;抗寒突变体
摘  要：
以彩叶凤梨继代苗为试材,通过甲基磺酸乙酯(EMS)诱变,研究了EMS处理对彩叶凤梨试管苗成活率及分化率的影响,同时以脯氨酸类似物L-羟脯氨酸(L-HYP)作为选择压力进行离体筛选,获得了彩叶凤梨抗羟脯氨酸的试管苗,并再生培养成抗性植株.结果表明,采用0.5%EMS对彩叶凤梨继代苗进行诱变处理6 h,其存活率和不定芽分化率分别为48.4%和36.9%;且7 mmol/L HYP筛选突变体植株效果最好,突变体植株在4℃低温胁迫下,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性增强,叶片电导率降低,对低温环境表现出一定抗性.
作  者：
CUI Keqiang;WANG Yanzun;SHI Huaping;WANG Jiping;Institute of Pomology,Shanxi Academy of Agricultural Sciences;College of Agronomy,Shanxi Agricultural University;
关键词：
Ethyl methane sulfonate;;Neoregelia caroline;;induced mutation;;cold resistance mutant
摘  要：
In this paper, the effect of EMS treatment on the survival rate and differentiation rate of tube seedlings in vitro from Neoregelia caroline was studied. And L-hydroxyproline(L-HYP)was used as selection stress to in vitro selection the mutants. Finally,hydroxyproline-resistant regenerated plants were obtained. The results showed that after 0.5% EMS treatment for 6 h, the survival rate and adventitious bud differentiation rate were 48.4% and 36.9%, respectively. The 7 mmol/L HYP screening mutants had the best effect,then the induced mutant could resist cold stress by increasing SOD and POD enzyme activities, decreasing conductivity under 4 ℃ low temperature stress.

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