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当前位置: 首页 > 文章 > MAR调控的胰岛素样生长因子-Ⅰ转化甘蓝的研究 园艺学报 2005,32 (2) 307-309
Position: Home > Articles > Transformation of Cabbage with Insulin-like Growth Factor-ⅠRegulated by Matrix Attachment Regions Acta Horticulturae Sinica 2005,32 (2) 307-309

MAR调控的胰岛素样生长因子-Ⅰ转化甘蓝的研究

作  者：
张应华;王小佳
单  位：
西南农业大学园林园艺学院
关键词：
胰岛素样生长因子-Ⅰ;核基质结合区;甘蓝;转化
摘  要：
采用根癌农杆菌介导法,将表达框两翼构建了核基质结合区(matrixattachmentregion,MAR)的胰岛素样生长因子Ⅰ(InsulinlikeGrowthFactorⅠ,IGFⅠ)基因导入甘蓝中,在含卡那霉素的培养基上连续4次筛选,通过10个批次的转化试验,获得25株抗性植株,PCR检测均呈阳性,随机选取5株进行基因组Southern杂交,结果有3株出现了杂交带,表明IGFⅠ基因已成功整合到甘蓝基因组中。
译  名：
Transformation of Cabbage with Insulin-like Growth Factor-ⅠRegulated by Matrix Attachment Regions
作  者：
Zhang Yinghua 1,2 and Wang Xiaojia 1* ( 1College of Horticulture and Landscape,Southwest Agricultural University,Key Lab in Vegetable Research of Chongqing, Chongqing 400716,China; 2College of Horticulture and Landscape,Yunnan Agricultural University,Kunming 650201, China)
关键词：
Insulin-like growth factor-Ⅰ; Matrix attachment region; Cabbage; Transformation
摘  要：
Hypocotyl explants of 7 day-old cabbage(Brassica oleracea L. var.capitata L.) seedlings were pre-cultured on bud induction medium(MS+BA 3 mg/L+NAA 0.1 mg/L)for two days. The explants were then infected with Agrobacterium tumefaciens strain EHA105 harboring a plasmid vector containing IGF-Ⅰ gene that flanked by matrix attachment regions(MARs)isolated from the genome of tobacco(Nicotiana tabacum L.). After two days of co-culture, the explants were transferred onto agrobacterium-suppressive medium(MS+BA 3mg/L+NAA 0.1 mg/L+Cb 400 mg/L) and cultivated for six days. Through four times of kanamycin-resistant selected culture(MS+BA 3 mg/L+NAA 0.1 mg/L+Km 5 mg/L+ Cb 400 mg/L),25 resistant shoots were obtained from 10 transformation experiments with total 15 200 explants. Some of the resistant regenerated plants were tested by PCR analysis and Southern blot, which showed IGF-Ⅰ gene was integrated into cabbage genome.

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