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Position: Home > Articles > Rapid Identification of Colletotrichum Spieces from Strawberry Hubei Agricultural Sciences 2010,49 (10) 2437-2438+2448

草莓炭疽病病原菌的快速鉴定

作  者:
吴金平;曾祥国;宋志红;向发云;吴娟;冯小明;顾玉成
单  位:
湖北省农业科学院经济作物研究所;安庆师范学院生命科学学院
关键词:
草莓炭疽病;鉴定;核糖体基因转录间隔区
摘  要:
草莓炭疽病近年来越来越严重,尤其在高温育苗阶段。为了减少该病害的危害,搞清楚其致病菌就显得尤为重要。试验利用PCR扩增真菌保守的ITS序列,确定其分类地位,避免了传统分类方法的繁琐、耗时和结果的不稳定性。结果表明,所收集的7个菌株中有6株为胶孢炭疽菌(Colletotrichum gloeosporioides),仅1株为尖孢炭疽菌(Colletotrichum acutatum)。
译  名:
Rapid Identification of Colletotrichum Spieces from Strawberry
作  者:
WU Jin-ping1,ZENG Xiang-guo1,SONG Zhi-hong1,XIANG Fa-yun1,WU Juan2,FENG Xiao-ming1,GU Yu-cheng1 (1.Institute Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064,China; 2.School of Life Science, Anqing Teachers College, Anqing 246011, Anhui, China)
关键词:
strawberry anthracnose; identification; rDNA internal transcribed spacer
摘  要:
Strawberry anthracnose is more and more serious in recent years, especially in the high-temperature propagation stage. In order to reduce threaten of this disease, it is important to understand the characteristic of the pathogen. With the rapid development of molecular biology, the taxonomic status was determined by PCR techniques which amplify the fungal ITS conservative sequences, avoiding tedious, time-consuming and unstable the traditional classification. The results showed that six strains were attributed to Colletotrichum gloeosporioides, only one strain was classified as Colletotrichum acutatum.

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