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当前位置: 首页 > 文章 > 利用EMS和炭疽病粗毒素诱变筛选草莓抗炭疽病突变体 北方园艺 2020 (1) 7-12
Position: Home > Articles > Screening of Strawberry Mutants Resistant to Anthracnose Disease Using Ethyl Methane Sulphonate (EMS) and Toxin Mutagenesis Techniques Northern Horticulture 2020 (1) 7-12

利用EMS和炭疽病粗毒素诱变筛选草莓抗炭疽病突变体

作  者：
张庆华;过聪;向发云;曾祥国;刘巧林;韩永超
单  位：
湖北省农业科学院经济作物研究所
关键词：
草莓炭疽病;EMS;粗提物;抗病突变体
摘  要：
以甲基磺酸乙酯(EMS)和暹罗炭疽菌(C.siamense)粗毒素为诱变剂,‘晶瑶’组培苗叶柄为原始材料,建立了抗炭疽病草莓材料诱变筛选体系,并筛选出了抗草莓炭疽病的突变体。结果表明:以0.8%的EMS溶液处理2 h可获得半致死效应;炭疽菌粗提物浓度为4%时为适宜的抗病选择压。对532个不定芽进行2次不连续离体筛选后获得43个抗性芽,成活率为8.1%。抗性芽再生植株移栽存活后,经炭疽菌离体接种鉴定,初步获得了抗炭疽病的草莓‘晶瑶’突变体株系13株,为后续进一步研究草莓炭疽病抗性机制提供了材料。
译  名：
Screening of Strawberry Mutants Resistant to Anthracnose Disease Using Ethyl Methane Sulphonate (EMS) and Toxin Mutagenesis Techniques
作  者：
ZHANG Qinghua;GUO Cong;XIANG Fayun;ZENG Xiangguo;LIU Qiaolin;HAN Yongchao;Institute of Industrial Crops,Hubei Academy of Agricultural Sciences;
关键词：
straberry anthracnose;;ethyl methane sulphonate(EMS);;crude extracts;;resistant mutants
摘  要：
The petioles of strawberry cultivar ‘Jingyao' in vitro were treated with chemical mutagen ethyl methane sulfonate(EMS)and the crude toxin produced by Colletotrichum siamense Nj-2,the pathogenic strain of straberry anthracnose.As a result,the system of in vitro screening some resistant mutants for strawberry anthracnose was established and the resistant mutants were obtained.The results showed that the petioles were treated with 0.8% of EMS for 2 hours which resulted in the effect of the ‘semi lethal dose',and the appropriate selection pressure was that the petioles were cultured in medium supplemented with 4% of Nj-2 crude extracts.There were 43 regenerated buds survival in 532 mutation and after twice discontinuous screening the survival rate was 8.1%,and of which 12 strains were identified as resistant mutants after inoculated with C.siamense isolate Nj-2 on leveas and petioles of mutans in vitro.This study provided materials for further study on the mechanism of strawberry anthracnose resistance.

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