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Position: Home > Articles > Detection of the expression and comparative study of miRNA - 17a * in HSN1 infected  and uninfected SPF ducks Heilongjiang Animal Science and Veterinary Medicine 2012 (11) 11-13

在感染与非感染H5N1亚型禽流感病毒状态下SPF鸭miRNA-17a水表达量检测及比较分析

作  者:
丛立新;宿甲子;孟继伦;邢沈阳;赵志辉;张金玉
单  位:
吉林大学畜牧兽医学院
关键词:
SPF鸭;miRNA-17a;H5N1亚型禽流感病毒;SPF duck; miRNA - 17a* ; HSN1 subtype of Avian influenza virus
摘  要:
为了探讨在H5N1亚型禽流感病毒感染状态下miRNA-17a’在水禽中的作用机制,试验应用茎环实时荧光定量PCR技术检测了miRNA-17a’在感染与非感染H5N1亚型禽流感病毒状态SPF鸭的不同组织中的相对表达。结果表明:在感染与非感染H5N1亚型禽流感病毒状态下SPF鸭的不同组织中均能检测到miRNA-17a’表达,在非感染状态下肺脏中相对表达量最高,感染状态下肌肉中相对表达量最高;感染H5N1亚型禽流感病毒后的SPF鸭与对照组SPF鸭相同组织间的相对比较分析显示,在感染状态下SPF鸭的气管中miRNA-17a’相对表达量是对照组SPF鸭的1.2倍,而在其他组织中其相对表达量低于对照组SPF鸭。
译  名:
Detection of the expression and comparative study of miRNA - 17a * in HSN1 infected  and uninfected SPF ducks
摘  要:
To explore the mechanism of action of miRNA - 17a * in waterfowl under the infection with the H5 N1 virus, the relative expressions of miRNA - 17a * in different tissues of the H5N1 - infected and uninfected SPF ducks were determined by stem - loop RT - PCR. The result showed that the expression of miRNA - 17a'* could be detected in the different tissues of infected and uninfected SPF duck. It was founded that the relative expression level of miRNA - 17a * in lung was highest in the uninfected SPF ducks, and there was a highest relative expression level in muscle of H5N1 infected ducks. The comparative analysis showed that the relative expression level of miRNA - 17a * in trachea of infected SPF ducks was 1.2 times than that in the SPF ducks of the control group, but the relative expression levels in other tissue were lower than that in the control group. 引用
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