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当前位置: 首页 > 文章 > 普洱茶茶色素提取工艺条件的响应面分析及其抗氧化性活性研究 食品科学 2011,32 (6) 1-6
Position: Home > Articles > Optimization of Process Conditions for Pu-erh Tea Pigment Extraction by Response Surface Analysis and Its Antioxidant Activity FOOD SCIENCE 2011,32 (6) 1-6

普洱茶茶色素提取工艺条件的响应面分析及其抗氧化性活性研究

作  者：
杨新河;李勤;黄建安;陆英;沈智;刘仲华
单  位：
国家植物功能成分利用工程技术研究中心
关键词：
普洱茶;茶色素;提取;响应面分析;抗氧化性活性
摘  要：
目的:优化普洱茶茶色素的提取工艺及研究其抗氧化性活性。方法:以与蒸馏水的色差作为普洱茶茶色素提取的评价指标,在单因素试验基础上,利用Box-Behnken中心组合试验和响应面分析法,对普洱茶茶色素提取工艺参数进行优化;以清除1,1-二苯基-2-苦基肼自由基(DPPH自由基)的能力评价普洱茶茶色素的抗氧化性活性。结果:普洱茶茶色素提取的最佳工艺条件为液料比45:1(mL/g)、提取温度100℃、提取时间150min、色差52.97,高出单因素试验和Box-Behnken组合中的最高色差4.73%;普洱茶茶色素清除DPPH自由基的IC50值30.83μg/mL。结论:采用响应曲面法对普洱茶茶色素提取条件进行优化可行;普洱茶茶色素具有良好的抗氧化作用,可作为天然抗氧化剂进一步开发和利用。
译  名：
Optimization of Process Conditions for Pu-erh Tea Pigment Extraction by Response Surface Analysis and Its Antioxidant Activity
作  者：
YANG Xin-he1,2,LI Qin1,3,HUANG Jian-an1,3,LU Ying1,3,SHEN Zhi1,LIU Zhong-hua1,3,(1.National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingedients,Changsha 410128,China;2.College of Life Science and Technology,Xiaogan University,Xiaogan 432000,China;3.Key Laboratory of Tea Science,Ministry of Education,Hunan Agricultural University,Changsha 410128,China)
关键词：
Pu-erh tea;tea pigment;extraction;response surface methodology;antioxidant activity
摘  要：
Objective:To optimize the extraction process for Pu-erh tea pigment and explore its antioxidant activity.Methods:Following single factor investigations,optimization of process conditions for the extraction of Pu-erh tea pigment based on the color difference value E between distilled water and Pu-erh tea pigment was carried out using central design composite design combined with response surface methodology(RSM).1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging assay was used to evaluate the antioxidant activity of the pigment.Results:The optimal extraction conditions for Pu-erh tea pigment were liquid-to-material ratio of 45:1(mL/g),extraction temperature of 100 ℃ and extraction time of 150 min.Under the optimal extraction conditions,a color difference E value of 52.97 was obtained,which revealed 4.73% increase as compared to the highest color difference in single-factor investigations and Box-Behnken composite experiments.The IC50 value for the scavenging capability of Pu-erh tea pigment on DPPH free radicals was 30.83μg/mL.Conclusion:It is feasible to optimize the extraction of Pu-erh tea pigment by response surface methodology.The Pu-erh tea pigment with excellent antioxidant activity has the potential to be further developed and utilized as a natural antioxidant.

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