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蚕蛹多糖脱蛋白方法研究

作  者:
伍善广;赖泰君;孙建华;刘旭辉;童张法
单  位:
河池学院化学与生命科学系;广西大学;广西大学化学化工学院
关键词:
蚕蛹;多糖;蛋白;脱除
摘  要:
以蛋白去除率和多糖损失率为指标,比较Sevag法、三氯乙酸(trichloroacetic acid,TCA)法、木瓜蛋白酶法、木瓜蛋白酶-Sevag联用法和木瓜蛋白酶-TCA联用法脱除蚕蛹多糖蛋白的效果。结果显示:木瓜蛋白酶-TCA联用效果最好,其脱蛋白的最佳条件为木瓜蛋白酶用量[E]/[S]2.0%、酶解温度50℃、酶解反应1.5h、采用TCA法除蛋白1次,此时蛋白去除率为97.98%,多糖损失率为18.76%;紫外光谱扫描定性实验显示脱蛋白的多糖在260~280nm处只有少量吸收,表明大多数蛋白已去除。木瓜蛋白酶-TCA联用法是一种有效的多糖脱蛋白方法。
译  名:
A Comparative Investigation into Deproteinization Methods for Silkworm Pupa Polysaccharides
作  者:
WU Shan-gang1,2,LAI Tai-jun1,SUN Jian-hua1,LIU Xu-hui1,3,TONG Zhang-fa1,LIAO Dan-kui1,*(1.College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China;2.Liuzhou Medical College,Liuzhou 545006,China;3.Department of Chemistry and Life Science,Hechi University,Yizhou 546300,China)
关键词:
pupa;polysaccharide;protein;removal
摘  要:
Protein removal rate and polysaccharide loss rate were used to evaluate and compare the effectiveness of Savag,s method,trichloroacetic acid(TCA) method,papain method and papain-TCA(papain hydrolysis followed by TCA precipitation) method in deproteinizing silkworm pupa polysaccharides.Papain-TCA method was found to be the best method for the deproteinization of silkworm pupa polysaccharides,and the optimal operating conditions were 1.5 h hydrolysis with papain at an enzyme-to-substrate of 2.0% and 50 ℃ followed by once treatment with TCA.A protein removal rate of 97.98% and a polysaccharide loss rate of 18.76% were achieved under such conditions.Only one absorption peak was observed in the UV-visible scanning spectrum of deporteinized silkworm pupa polysaccharides,indicating the removal of almost all proteins.

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