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Position: Home > Articles > Isolation and Purification of Total Flavonoids from Acanthaceae by AB-8 Resin Hubei Agricultural Sciences 2009,48 (12) 221-224

AB-8大孔树脂分离纯化爵床总黄酮的研究

作  者:
周燕芳;丁冬纯
单  位:
韩山师范学院化学系
关键词:
AB-8大孔树脂;爵床;总黄酮;分离纯化
摘  要:
用AB-8大孔吸附树脂对爵床总黄酮进行分离纯化。结果表明,静态分离纯化的最佳工艺参数为提取液中总黄酮量在2.0~3.5mg/mL范围,AB-8树脂与提取液以1∶20(W/V)的比例,于25℃,在酸性或碱性的条件下恒温振荡24h后,用90%的乙醇洗脱。此条件下总黄酮的得率可达38.24%。动态分离纯化的最佳工艺参数为流速1.0mL/min,粗提液中总黄酮量在1.5~2.5mg/mL范围,在pH值为2的条件下总黄酮的得率为42.72%。
译  名:
Isolation and Purification of Total Flavonoids from Acanthaceae by AB-8 Resin
作  者:
ZHOU Yan-fang,DING Dong-chun (Deparment of Chemistry,Hanshan Normal University,Chaozhou 521041,Guangdong,China)
关键词:
AB-8 resin; acanthaceae; total flavonoids; isolation and purification
摘  要:
The extraction and purification of the flavonoid from acanthaceae was studied by AB-8 resin. The results showed that the effect of dynamic purification of acanthaceae was better than the static purification. The best process parameters for dynamic isolation and purification were as follows: flow rate was 1mL/min, flavonoid contents of crude extract were 1.5~2.5mg/mL,pH was 2,the purification yield was 42.72%.

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