中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 超声波辅助提取植物油中甾醇和生育酚 中国油料作物学报 2016,38 (3) 373-378
Position: Home > Articles > Ultrasound-assisted extraction of sterols and tocopherol from edible oils Chinese Journal of Oil Crop Sciences 2016,38 (3) 373-378

超声波辅助提取植物油中甾醇和生育酚

作  者：
王素君;李培武;张良晓;王秀嫔;张文;朱建国;陈小媚;张奇
单  位：
中国农业科学院油料作物研究所
关键词：
植物油;豆甾醇;β-谷甾醇;δ-生育酚;中心组合设计;高效液相色谱串联质谱法
摘  要：
为优化食用植物油中主要营养物质的定量分析方法,建立了超声波辅助萃取LC-MS/MS测定食用油中植物甾醇和生育酚分析方法。在单因素实验的基础上,采取中心组合设计优化实验中超声时间和超声温度。通过优化分析得到食用油皂化提取豆甾醇、β-谷甾醇和δ-生育酚最优实验条件为:超声反应温度75℃,超声反应时间40min,功率100%。本方法中豆甾醇、β-谷甾醇、δ-生育酚的定量限分别为10.0ng/m L、10.0ng/m L和8.1ng/m L,相关系数均≥0.990 4,加标回收率在87.2%~97.8%之间,相对标准偏差为2.3%~8.6%。将建立的方法应用于茶籽油、紫苏子油、橄榄油、大豆油样品分析,结果表明本方法前处理操作简单且用时短、结果准确可靠,适用于食用植物油中主要甾醇和生育酚定量分析。
译  名：
Ultrasound-assisted extraction of sterols and tocopherol from edible oils
作  者：
WANG Su-jun;LI Pei-wu;ZHANG Liang-xiao;WANG Xiu-pin;ZHANG Wen;ZHU Jian-guo;CHEN Xiao-mei;ZHANG Qi;Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences;Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture;Laboratory of Risk Assessment for Oilseed Products ( Wuhan) ,Ministry of Agriculture;Key Laboratory of Detection for Mycotoxins,Ministry of Agriculture;Quality Inspection and Test Center for Oilseed Products,Ministry of Agriculture;
关键词：
Vegetable oil;;Stigmasterol;;β-sitosterol;;δ-tocopherol;;Central composite design;;LC-MS/MS
摘  要：
To optimize nutriment quantitative analysis in eadible oil,we developed an LC- MS / MS method coupled with ultrasonic extraction to analyze phytosterol and tocopherol. Central composite design was employed to optimize the extraction conditions including ultrasonic time and ultrasonic temperature. After systemic analysis,the optimized experimental conditions were obtained as follows: ultrasonic temperature at 75℃,ultrasonic time of 40 min and ultrasonic power of 100%. Validation indicated that the limits of detection( LOQ) were 10. 0 ng / m L,10. 0 ng / m L and 8. 1 ng / m L for stigmasterol,β- sitosterol and δ- tocopherol respectively. Recoveries ranged from 87. 2% to 97. 8%. Correlation coefficients were higher than 0. 9904. And the relative standard deviations( RSD) were 2. 3% to 8. 6%. Consequently the method was used to analyze stigmasterol,β- sitosterol and δ- tocopherol in camellia oil,petilla oil,olive oil and soybean oil. Results showed that this method was simple,rapid,accurate and reliable,which was a suitable method for quantitative analysis of main phytosterols and tocopherols in vegetable oil.

相似文章

导出引用
计量
文章访问数: 17
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46