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当前位置: 首页 > 文章 > 微囊藻毒素多克隆抗体的纯化及F(ab′)_2片段的制备 江苏农业学报 2010,26 (3) 199-202
Position: Home > Articles > Purification of Polyclonal-antibodies against Microcystin-LR and Preparation of F(ab′)_2 Fragments Jiangsu Journal of Agricultural Sciences 2010,26 (3) 199-202

微囊藻毒素多克隆抗体的纯化及F(ab′)_2片段的制备

作  者：
纪炜;刘媛;刘贤进
单  位：
南京农业大学植物保护学院;江苏省农业科学院食品质量安全检测研究所
关键词：
多克隆抗体;木瓜蛋白酶;离子交换色谱;F(ab′)2片段
摘  要：
为制备微囊藻毒素(MCYST-LR)多克隆抗体F(ab′)2片段的方法。采用Protein G亲和柱纯化微囊藻毒素多克隆抗体,经木瓜蛋白酶在温和条件下酶切抗体,利用DEAE-52离子交换树脂柱分离抗体酶切片段,获得F(ab′)2片段。SDS-PAGE分析表明,MCYST-LR多克隆抗体得到有效纯化,木瓜蛋白酶酶切后出现明显F(ab′)2片段的对应谱带;利用紫外分光光度计法测得F(ab′)2片段的产率为30.6%。采用木瓜蛋白酶法成功制备了MCYST-LR多克隆抗体的F(ab′)2片段。
译  名：
Purification of Polyclonal-antibodies against Microcystin-LR and Preparation of F(ab′)_2 Fragments
作  者：
JI Wei1,2,LIU Yuan2,LIU Xian-jin2(1.College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Food Quality Safety and Detection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
关键词：
polyclonal-antibody;papain;ion-exchange chromatography;F(ab′)2 fragment
摘  要：
The polyantibodies against microcystin-LR were purified using affinity chromatography with Protein G.The purified polyclonal antibodies were digested by papain under mild conditions.The antibody fragments were separated by DEAE-52 ion-exchange chromatography to retrieve the F(ab′)2 fragments.The SDS-PAGE results showed that the microcystin-LR polyclonal antibodies effectively purified by using Protein G affinity chromatography released the F(ab′)2 fragments from papain-digested microcystin-LR polyclonal antibodies.The yield rate of F(ab′)2 fragments was 30.6% by UV-spectrophotometer.F(ab′)2 fragments of polyclonal antibodies against MCYST-LR were produced successfully by papain enzymolysis method.

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