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Rep-PCR技术及其应用现状

作  者:
赵燕梅;张吉明;许庆方
单  位:
山西农业大学动物科技学院
关键词:
Rep-PCR;细菌;真菌;环境微生物;遗传多样性
摘  要:
文章着重介绍了Rep-PCR技术原理、特点及其在细菌、真菌、环境微生物遗传多样性研究方面的应用。Rep-PCR目前发展迅速并被广泛应用于多种细菌基因分类,此方法操作方便,可以大样本进行,且Rep-PCR分辨效果好,可重复性强。现在Rep-PCR可自动化分型,并可建立各种细菌REP、ERIC-PCR分型标准数据库。但Rep-PCR也存在一定问题,不同来源的或不同批次的引物和酶,对Rep-PCR结果都有一定的影响。
译  名:
Application Status of Rep-PCR Technology
作  者:
Zhao Yan-mei;Zhang Ji-ming;Xu Qing-fang;College of Animal Science and Technology,Shanxi Agricultural University;
关键词:
Rep-PCR;;bacteria;;fungi;;environmental microbial;;genetic diversity
摘  要:
This paper focuses on the principle,characteristics of Rep-PCR and its applications in bacteria,fungi,environmental microbial genetic diversity. Rep-PCR has been developed rapidly and applied widely in gene classification of bacteria,fungi,environmental microbial. The method can analyse large sample with conveniently. Besides,the Rep-PCR has a good resolution and repeatability. Currently,Rep-PCR is available to automatic classification,and establishing classification standard database of REP,ERIC-PCR. But Rep-PCR also has some problems,different sources or different batches of primers and enzymes have a certain influence on the result of Rep-PCR.

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