中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 马铃薯MYB转录因子基因的序列分析 分子植物育种 2019 (2) 6596-6603
Position: Home > Articles > 马铃薯MYB转录因子基因的序列分析 Molecular Plant Breeding 2019 (2) 6596-6603

马铃薯MYB转录因子基因的序列分析

作  者：
刘旭婷;范菠菠;张旭婷;王鹏伟;高慧;马艳红
单  位：
内蒙古农业大学农学院
关键词：
马铃薯(Solanum tuberosum);MYB;转录因子;序列分析
摘  要：
MYB基因家族是植物中最大的一类转录因子家族,广泛参与植物的生长发育和代谢调控过程.本研究通过生物信息学方法对美国国立生物技术信息中心(national center for biotechnology information, NCBI)中筛选的16条马铃薯MYB基因进行分析,预测其分子量和等电点等理化性质、基序、二级结构和三级结构,并用MEGA 5.0与已报道具有明确功能的20个MYB蛋白进行多序列比对分析并构建系统进化树.结果表明:马铃薯中筛选出16个的MYB基因蛋白大小在104~379 aa,分子量和等电点分别介于66 877.11~147 918.09 k D和4.95~5.16之间;基序预测得到3个特征基序,最长的基序含50个氨基酸;α-螺旋和无规则卷曲是主要的二级结构,三级结构中都含有螺旋-转角-螺旋结构;进化树分析中,其中StMYB12与SIMYB12同源性高,StMYBA1、St AN1和StMYB113与矮牵牛AN2同源性高,StMYB21与MdMYB1有同源性,推测马铃薯的St MYB12、St MYBA1、StAN2、St AN1、StMYB113和StMYB21可能参与花青素合成代谢调控,这为下一步马铃薯花色素苷合成调控相关MYB基因功能研究提供参考依据.
作  者：
Liu Xuting;Fan Bobo;Zhang Xuting;Wang Pengwei;Gao Hui;Ma Yanhong;Agronomy College,Inner Mongolia Agricultural University;
关键词：
Solanum tuberosum L.;;MYB;;Transcription factor;;Sequence analysis
摘  要：
The MYB gene family is the largest family of transcription factors in plants, and is widely involved in plant growth and development and metabolic regulation. In this study, 16 MYB genes of Solanum tuberosum that were screened from National Center for Biotechnology Information(NCBI) analyzed by biological methods. Its predicted that molecular weight, isoelectric point, physical and chemical properties, motif, two level structure and three level structure. And multiple sequence alignment analysis and construction of phylogenetic tree were performed with 20 MYB proteins with clear functions via MEGA 5. The results show that the protein size of 16 MYB genes from Solanum tuberosum was range from 104 aa to 379 aa, and the molecular weight and isoelectric point were 66 877.11～147 918.09 kD and 4.95～5.16, respectively. The base order is predicted by 3 characteristic order, the longest sequence contains 50 amino acids, the alpha helix and the irregular curl are the main two structure, and the three grade structure contains spiral-angle-helix structure. In the phylogenetic tree analysis,StMYB12 has high homology with SIMYB12, St MYBA1, StAN1 and StMYB113 have high homology with Petunia AN2, StMYB21 has homology with MdMYB1. It is speculated that StMYB12, StMYBA1, StAN2,St AN1, StMYB113 and St MYB21 of S. tuberosum may be involved in anabolic regulation of anthocyanin. This study provides a reference for further study on the function of MYB gene in S. tuberosum.

相似文章

导出引用
计量
文章访问数: 19
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46