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Position: Home > Articles > 利用酵母双杂交技术筛选棉铃虫触角内与G蛋白q家族α亚基(Gqα)互作的蛋白(英文) Acta Entomologica Sinica 2010,53 (12) 1339-1344

Screening of proteins interacting with Gqα by yeast two-hybrid technique in the antenna of Helicoverpa armigera(Lepidoptera:Noctuidae)

作者：

Su Honghua;Wang Guirong;Zhang Yongjun;Liang Gemei;Wu Kongming;Guo Yuyuan

单位：

Institute of Plant Protection

关键词：

Helicoverpa armigera;Gqα;antenna cDNA library;yeast two-hybrid technique;signal transduction;pheromone binding protein

摘要：

G proteins are important signalling components. Many findings indicated that members of Gq family were functionally diverse than previously thought. In order to determine the exact functions of the α subunit of Gq family of G proteins (Gqα) in signal transduction pathway, Gqα was used as the bait to screen the antenna-cDNA library of the cotton bollworm Helicoverpa armigera by the two-hybrid system. More than ten proteins including pheromone binding protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein S20, cytochrome oxidase subunit Ⅰ, allatotropin neuropeptide precursor (AT2), translationally controlled tumor protein (TCTP), humoral lectin prepropeptide and glycosylasparaginase were found to interact with Gqα, suggesting that Gqα plays diverse roles in signal transduction. Among the diverse functions, Gqα is most likely to be involved in the pheromone detection among olfactory signalling. The findings may provide a primary basis for future construction of an integrated signalling network in the moth

译名：

利用酵母双杂交技术筛选棉铃虫触角内与G蛋白q家族α亚基(Gqα)互作的蛋白(英文)

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Screening of proteins interacting with Gqα by yeast two-hybrid technique in the antenna of Helicoverpa armigera(Lepidoptera:Noctuidae)

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