作 者：
胡凌豪;葛俊苗;宋益善;陈建康;盛洁;李路瑶;金礼;雷洲
单 位：
国家淡水水产品加工技术研发分中心(上海);上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心
摘 要：
为了优化凤尾鱼酶解工艺并研究其酶解产物促嗜热链球菌增殖作用。在单因素实验基础上,以酶解物对嗜热链球菌的增殖效果为评价指标,运用单因素试验设计及响应面分析方法优化了风味蛋白酶对凤尾鱼的酶解工艺条件。结果显示,最佳酶解条件为:酶解时间5.4 h,料液比1∶2.2 g/mL,加酶量5.4%;此条件下酶解产物促进嗜热链球菌增殖效果ΔOD600为0.556。同时,对不同分子量段的酶解产物促进嗜热链球菌增殖情况进行研究,结果表明分子量小于3000Da的酶解产物促进嗜热链球菌增殖效果最优,其增殖效果ΔOD600达到0.556;另外,此分子量段酶解产物在培养基中的最佳含量为1.2 g/L。抗氧化性实验结果表明,小于3000Da的酶解产物对DPPH~+·的清除率达70%,IC_(50)值为2.73 mg/mL。
译 名：
Optimization on Enzymolysis Conditions and the Proliferation Effect on Streptococcus thermophiles Growth by the Anchovy Hydrolysate
作 者：
HU Ling-hao;GE Jun-miao;SONG Yi-shan;CHEN Jian-kang;SHENG Jie;LI Lu-yao;JIN Li;LEI Zhou;College of Food Science and Technology,Shanghai Ocean University;Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation;National R&D Branch Center for Freshwater Aquatic Products Processing Technology( Shanghai);
关键词：
anchovy;;enzymatic hydrolysis;;Streptococcus thermophiles;;proliferation;;response surface methodology
摘 要：
This study aims to optimize the enzymatic hydrolysis process of anchovy and study the effect on proliferation of Streptococcus thermophiles by the anchovy hydrolysate.Basing on single factor experiments,the response surface methodology( RSM) was employed to optimize the enzymatic hydrolysiscondition of anchovy,by using proliferation effect on Streptococcus thermophilus growth as the evaluation index.The results showed that the optimum conditions were hydrolysis time 5.4 h,the ratio of raw material to water 1∶ 2.2( g/mL) and enzyme concentration 5.4%,and under these conditions,the ΔOD600 of proliferated Streptococcus thermophilus was 0.556. The effect of different molecular weight segments on the proliferation of streptococcus thermophilus was studied,the results showed that the fragments with molecular weights of less than 3000 Da,significantly promoted the growth of Streptococcus thermophiles,moreover,the optimum content of the fragment in the growth medium was1.2 g/L. The results of antioxidation experiments showed that the highest scavenging capacity of fragments with molecular weights of less than 3000 Da against DPPH~+· was 70% respectively,and IC_(50) value was about 2.73 mg/mL.
