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Position: Home > Articles > Clone and sequence analysis of SLA-DQB gene of Guangxi Bama mini-pig Guangdong Agricultural Sciences 2014,41 (5) 187-189+193

广西巴马小型猪SLA-DQB基因的克隆及序列分析

作  者:
霍秋红;杨秀荣;黄光成;黄化平;顾浩;兰干球;蒋和生;蒋钦杨;郭亚芬
单  位:
广西大学动物科学技术学院
关键词:
广西巴马小型猪;SLA-DQB;异种器官移植;免疫排斥
摘  要:
利用RT-PCR的方法从广西巴马小型猪肝脏组织中扩增SLA-DQB基因的编码区序列,旨在为下一步构建SLA-DQB转基因广西巴马小型猪奠定基础。结果表明,克隆的广西巴马小型猪SLA-DQB基因编码区长687 bp,与GenBank参考序列(NM_001113694)相比,共有15个氨基酸发生突变(位点为10、14、19、27、29、38、39、48、58、61、68、72、76、78、183)。与普通猪、其他小型猪、奶牛、人类及小鼠的同源性分别为95.8%、95.8%、88.1%、86.2%和80.0%。
译  名:
Clone and sequence analysis of SLA-DQB gene of Guangxi Bama mini-pig
作  者:
HUO Qiu-hong;YANG Xiu-rong;HUANG Guang-cheng;HUANG Hua-ping;GU Hao;LAN Gan-qiu;JIANG He-sheng;JIANG Qin-yang;GUO Ya-fen;College of Animal Science and Technology, Guangxi University;
关键词:
Guangxi Bama mini-pig;;SLA-DQB;;xenotransplantation;;immune rejection
摘  要:
Reverse transcription(RT) PCR was used to amplify the coding sequence of SLA-DQB in liver from Guangxi Bama mini-pig in order to prepare for SLA-DQB transgenic Guangxi Bama mini-pig. The result showed that the length of SLA-DQB was 687 bp. 15 missense mutations(Site 10, 14, 19, 27, 29, 38, 39, 48, 58, 61, 68, 72, 76, 78, 183) were found and led to the change of amino acid in SLA-DQB in Guangxi Bama mini-pig compared with reference sequence(NM_001113694). Homological analysis showed that there were 95.8%, 95.8%, 88.1%, 86.2%, 80.0% compared with Sus scrofa, miniature swine, Bos taurus, Homo sapiens, and Mus musculus, respectively.

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