当前位置: 首页 > 文章 > 腺苷产生菌Xn151菌株的选育及发酵条件研究 生物技术通报 2008,0 (1) 425-430
Position: Home > Articles > Breeding of and Fermentation an Adenosine-producing Strain Xn151 Biotechnology Bulletin 2008,0 (1) 425-430

腺苷产生菌Xn151菌株的选育及发酵条件研究

作  者:
施庆珊;林小平;许虹;疏秀林;冯静;陈爱美;邱玉棠;欧阳友生;陈仪本
单  位:
广东省微生物研究所广东省微生物应用新技术公共实验室
关键词:
腺苷;菌种选育;发酵;正交实验设计
摘  要:
以肌苷产生菌枯草杆菌GMI-741(Ade~(--))为出发菌株,通过多因子诱变选育出具有黄嘌呤、鸟嘌呤双重营养缺陷型、丧失腺嘌呤脱氨酶的腺苷产生菌Xn151。以Xn151为发酵菌株,采用正交实验设计法对其发酵基础培养基进行优化,确定最佳发酵配方。利用此培养及配方摇瓶发酵72h,腺苷产量可达12.3g/L。
译  名:
Breeding of and Fermentation an Adenosine-producing Strain Xn151
作  者:
Shi Qingshan Lin Xiaoping Xu Hong Shu Xiulin Feng Jing Chen Aimei Qiu Yutang OuYang Yousheng Chen Yiben (Guangdong Institute of Microbiology,Guangdong Open Laboratory of Applied Microbiology,Guangzhou 510070)
关键词:
Adenosine;;Strain breeding;;Fermentation;;Orthogonal factorial design
摘  要:
The induction of adenosine-producing strain from an inosine-producing strain Bacillus substilis GMI-741 was attempted,and it was found out that a diauxotrophi mutant(xanthine and guanine-requiring)Bacillus substilis Xn151 lacking of adenase produce a large amount of adenosine.The orthogonal factorial design was carried out,to optimize the culture medium.The optimal medium,based on statistical analysis,showed a production of 12.3 g/L adenosine on a reciprocal shaker for 72 h.

相似文章

计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊