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当前位置: 首页 > 文章 > 蛹虫草退化菌株的生理生化特征 食用菌学报 2019 (4) 23-28+157
Position: Home > Articles > Physiological and Biochemical Characteristics of a Degraded Strain of Cordyceps militaris Acta Edulis Fungi 2019 (4) 23-28+157

蛹虫草退化菌株的生理生化特征

作  者：
周思池;邹根;杨占山;鲍大鹏;姚威威;杨杰;汪滢;李晓玲
单  位：
长春工业大学化学与生命科学学院;徐州鸿宇农业科技有限公司;上海市农业科学院食用菌研究所农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心国家食用菌加工技术研发分中心上海市农业遗传育种重点开放实验室
关键词：
蛹虫草;菌株退化;腺苷;虫草素;脂质;自噬相关基因
摘  要：
为了初步鉴定蛹虫草(Cordyceps militaris)退化菌株的生理生化特征,采用倒置显微镜、扫描电子显微镜观察蛹虫草退化菌株与正常菌株的形态特征,分别用高效液相色谱(HPLC)、氟硼二吡咯(BODIPY)脂滴染色、荧光定量PCR测定腺苷与虫草素含量、脂质含量、细胞自噬相关基因的表达水平。结果表明:蛹虫草退化菌株的菌丝体分枝多、畸形,呈弯曲的不规则状;退化菌株的菌丝中腺苷含量比正常菌株的低30.23%,两者发酵液中的腺苷含量无差异,而退化菌株的菌丝和发酵液中均未检测到虫草素;退化菌株的荧光亮度明显低于正常菌株;细胞自噬相关基因Atg13、Atg18、Atg22、Atg22-2表达量在退化菌株中上升,退化菌株比正常菌株分别上升了432.19%、170.45%、336.06%、61.01%;Atg17呈下调表达,退化菌株比正常菌株下调了65.28%,两者Atg28的表达量无差异。
译  名：
Physiological and Biochemical Characteristics of a Degraded Strain of Cordyceps militaris
作  者：
ZHOU Sichi;ZOU Gen;YANG Zhanshan;BAO Dapeng;YAO Weiwei;YANG Jie;WANG Yin;LI Xiaolin;School of Chemistry and Life Science,Changchun University of Technology;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences;Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture,P.R.China;National Engineering Research Center of Edible Fungi;National R&D Center for Edible Fungi Processing;Key Laboratory of Agricultural Genetics and Breeding of Shanghai;Xuzhou Hongyu Agricultural Technology Co.,Ltd;
关键词：
Cordyceps militaris;;culture degeneration;;adenosine;;cordycepin;;lipid;;autophagy related gene
摘  要：
A preliminary study was carried out to identify physiological and biochemical characteristics of a degraded strain of Cordyceps militaris, Dene3. The morphological characteristics of Dene3 and those of the normal strain were observed by both inverted microscopy and scanning electron microscopy. Adenosine and cordycepin content were determined by high performance liquid chromatography(HPLC); lipid content was determined by the BODIPY lipid droplet staining method; and expression levels of autophagy-related genes in C. militaris was determined by real-time PCR. The results showed that the mycelia of the degraded strain Dene3 were deformed, irregularly curved with many branches. Compared to the normal strain, Dene3 contained 30.23% less adenosine in its hyphae. There was no difference in adenosine content between the fermentation broth of Dene3 and that of the normal strain. Cordycepin was not detected in either the fermentation broth or the mycelia of Dene3. Dene3 showed significantly lower fluorescence intensity than the normal strain. Compared to the normal strain, autophagy-related genes Atg13, Atg18, Atg22 and Atg22-2 were up-regulated in Dene3 by 432.19%, 170.45%, 336.06%, and 61.01%, respectively; and Atg17 was down-regulated in Dene3 by 65.28%. There was no difference in the expression of Atg28 between Dene3 and the normal strain.

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