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当前位置: 首页 > 文章 > 反相高效液相色谱法测定蛹虫草中腺苷和虫草素的含量 食品科学 2010,31 (16) 172-175
Position: Home > Articles > Analysis of Adenosine and Cordycepin in Cordyceps militaris (L.) Link by Reversed Phase-High Performance Liquid Chromatography FOOD SCIENCE 2010,31 (16) 172-175

反相高效液相色谱法测定蛹虫草中腺苷和虫草素的含量

作  者：
刘小芳;薛长湖;常耀光
单  位：
中国海洋大学食品科学与工程学院
关键词：
反相高效液相色谱法;蛹虫草;腺苷;虫草素
摘  要：
建立一种新的反相高效液相色谱方法,用于蛹虫草中腺苷和虫草素的定性定量分析。样品经水超声提取,以终浓度20mmol/L柠檬酸、40mmol/L醋酸、20mmol/L三乙胺的混合溶液-乙腈(体积比98:2)为流动相,流速1.0mL/min,色谱柱CapcellpakC18(150mm×4.6mm,5μm)分离,柱温40℃,进样量10μL,紫外检测波长260nm。在此条件下,腺苷在1～1000μg/mL范围内有良好的线性关系(Y=33574X+54.526,R2=0.9999),检测限0.2μg/mL,回收率93.75%～96.90%,RSD为0.116%;虫草素在2～1000μg/mL范围内有良好的线性关系(Y=34385X+23.103,R2=1.0000),检测限0.2μg/mL,回收率92.03%～97.35%,RSD为0.104%。该方法样品处理简便、分离度高、杂质干扰小、回收率高、重复性好,能够对蛹虫草中腺苷和虫草素进行准确的定性定量分析。
译  名：
Analysis of Adenosine and Cordycepin in Cordyceps militaris (L.) Link by Reversed Phase-High Performance Liquid Chromatography
作  者：
LIU Xiao-fang,XUE Chang-hu*,CHANG Yao-guang (College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)
关键词：
reversed phase-high performance liquid chromatography;Cordyceps militaris ( L.) Link;adenosine;cordycepin
摘  要：
In this paper, a RP-HPLC method for determining the contents of adenosine and cordycepin in Cordyceps militaris ( L.) Link is described. The separation column was Capcellpak C18 (150 mm × 4.6 mm, 5μm), which was held at 40 ℃. The mobile phase was composed of a mixed solution containing 20 mmol/L citric acid, 40 mmol/L acetic acid and 20 mmol/L triethylamine and acetonitrile (V/V, 98:2) at a flow rate of 1.0 mL/min. The sample loading volume was 10μL. UV detection was performed at 260 nm. The method showed a good linear relationship between peak area and concentration over the range from 1 to 1000μg/mL (R2 = 0.9999) for adenosine and from 2 to 1000μg/mL (R2 = 1.0000) for cordycepin. The spike recoveries of adenosine in 3 replicates determinations ranged from 93.75% to 96.90% and those of cordycepin from 92.03% to 97.35%.The limit of detection was 0.2 μg/mL for both adenosine and cordycepin. The RSD of precision was 0.116% for adenosine and 0.104% for cordycepin. This method is characterized by simplicity and high sensitivity and accuracy.

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