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当前位置: 首页 > 文章 > 用于低乳糖牛奶生产的乳糖酶添加剂候选菌株筛选及粗酶性质研究 河南农业科学 2011,40 (4) 138-141+145
Position: Home > Articles > Screening of A Psychrotolerant Bacterium of Planococcus sp. Y Producing Low Temperature Lactose and Preliminary Study on Its Enzyme Activity Journal of Henan Agricultural Sciences 2011,40 (4) 138-141+145

用于低乳糖牛奶生产的乳糖酶添加剂候选菌株筛选及粗酶性质研究

作  者：
李荷;张博;张敏文;顾取良
单  位：
广东药学院生物化学与分子生物学系
关键词：
乳糖酶;筛选;低温菌
摘  要：
在低乳糖牛奶加工过程中需要高活性的低温乳糖酶作为添加剂。为此,从采集土样中分离纯化获得1株低乳糖奶加工过程中需要的高乳糖酶活性候选菌株,应用形态学和分子生物学方法,初步鉴定该菌株为动球菌属(Planococcus sp.),命名为Planococcus sp.Y。研究表明,该菌株是1株低温菌株,其最适生长温度为25℃,最佳生长pH值为7.0,所产酶为胞内酶,粗酶液的最适酸碱度和温度分别为pH7.0和25℃。
译  名：
Screening of A Psychrotolerant Bacterium of Planococcus sp. Y Producing Low Temperature Lactose and Preliminary Study on Its Enzyme Activity
作  者：
LI He,ZHANG Bo,ZHANG Min-wen,GU Qu-liang(Department of Biochemistry&Molecular Biology,Guangdong Pharmaceutical University,Guangzhou 510006,China)
关键词：
Lactase;Screening;Psychrotolerant bacterium
摘  要：
High activity of cold-adapted low lactase as an additive is necessary in processing low lactose milk.The psychrotrophic and bacterium strain was isolated from surficial soils.A strain capable of producing psychrotrophic a lactase was identified as a Planococcus sp.Y according to the morphological and molecular characteristics.The optimal conditions for the growth of the strain were examined.The optimal temperature and pH value for growth were 25℃ and 7.0,respectively.Study on the activity of the lactase showed that the optimal temperature and pH for the activity of the crude enzyme was 25℃ and 7.0,respectively.The lactase from Planococcus sp.Y was located intracellularly.This will provide a foundation for producing low lactose milk in future.

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