中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 基于超滤法从牛初乳中分离胰岛素样生长因子-Ⅰ的工艺条件 食品科学 2010,31 (22) 86-90
Position: Home > Articles > Use of Ultra-filtration for Separation of Insulin-like Growth Factor-Ⅰfrom Bovine Colostrums FOOD SCIENCE 2010,31 (22) 86-90

基于超滤法从牛初乳中分离胰岛素样生长因子-Ⅰ的工艺条件

作  者：
郭洪;陈庆森;闫亚丽
单  位：
天津市食品生物技术重点实验室天津商业大学生物技术与食品科学学院
关键词：
牛初乳;胰岛素样生长因子-Ⅰ(IGF-Ⅰ);脱脂;超滤法;工艺条件
摘  要：
利用超滤技术从牛初乳中分离IGF-Ⅰ,并对脱脂和超滤条件进行优化。IGF-Ⅰ的质量浓度检测采用放射免疫法测定,总蛋白质量浓度采用考马斯亮蓝法测定。结果表明:牛初乳中分离IGF-Ⅰ过程中制备脱脂乳最适工艺条件为36℃、4000r/min离心15min;经截留分子质量为10～20kD的中空纤维膜超滤除杂蛋白和6kD的中空纤维膜超滤去除乳糖等小分子物质,所得的IGF-Ⅰ的纯度浓度较初乳中提高了约3660倍。本方法确立了从牛初乳中分离纯化较高纯度IGF-Ⅰ的技术路线。
译  名：
Use of Ultra-filtration for Separation of Insulin-like Growth Factor-Ⅰfrom Bovine Colostrums
作  者：
GUO Hong,CHEN Qing-sen*,YAN Ya-li (Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China)
关键词：
colostrums;insulin-like growth factorⅠ(IGFⅠ);lipid extraction;ultra-filtration;technological conditions
摘  要：
Insulin-like growth factors play important roles in the cell differentiation, animal growth, development, reproduction and metabolism, and are largely present in the bovine colostrums. In present study, insulin-like growth factorⅠ(IGFⅠ) was separated from bovine colostrums by using ultra-filtration, and the degreasing and ultra-filtration conditions were optimized. The IGF-Ⅰand total protein concentrations were detected by radio-immunoassay(RIA) and coomassie blue staining, respectively. The results showed that the optimum degreasing conditions were as follows: centrifugation temperature 36 ℃, centrifugation speed 4000 r/min and centrifugation time 15 min. Macromolecular impurities protein and lactose could be removed after ultra-filtration with molecular weight cut-off 10-20 kD and 6 kD membrane, respectively. The relative concentration of IGF-Ⅰwas 3660 times higher than that in colostrums after ultra-filtration.

相似文章

导出引用
计量
文章访问数: 3
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46