当前位置: 首页 > 文章 > 变性梯度凝胶电泳技术及其在食品微生物研究中的应用 食品科学 2009,30 (11) 266-272
Position: Home > Articles > Review on Denaturing Gradient Gel Electrophoresis and Its Application in Food Microbiology FOOD SCIENCE 2009,30 (11) 266-272

变性梯度凝胶电泳技术及其在食品微生物研究中的应用

作  者:
刘灿;生吉萍;申琳
单  位:
中国农业大学食品科学与营养工程学院
关键词:
食品微生物;变性梯度凝胶电泳(DGGE);微生物多样性
摘  要:
食品中的很多微生物无法分离培养,传统的微生物研究方法不仅费时费力,而且会造成偏差。变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)是一种不依赖于纯培养的分子技术,因其快速、灵敏、分析全面等特点已被广泛应用于微生物生态学的研究中。本文介绍了DGGE的发展历程、原理、操作步骤及其在食品微生物研究中的进展,评价了该技术的优势和局限性,展望了其在食品领域内的应用前景。
译  名:
Review on Denaturing Gradient Gel Electrophoresis and Its Application in Food Microbiology
作  者:
LIU Can1,SHENG Ji-ping1,SHEN Lin1,2,* (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China ; 2. College of Food Science, Xinjiang Agricultural University, Urumqi 830052, China)
关键词:
food microorganism;denaturing gradient gel electrophoresis (DGGE);microbial diversity
摘  要:
There are lots of unknown and "unculturable" microorganisms in food, but traditional microbiological methods not only are labor-intensive and time-consuming, but may cause deviation. Denaturing gradient gel electrophoresis (DGGE) is a cultural-independent molecular technique, and has been recently introduced into food microbial ecology. DGGE could easily identify the microorganisms from food, avoiding the potential deviation of traditional methods. This review summarized the development, concept, principle, operation procedure of DGGE and its application in food and food-related ecosystems. The limitation and application prospect of this approach in food microbiology were also discussed.

相似文章

计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊