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当前位置: 首页 > 文章 > 京海黄鸡Myf5基因表达谱及表达规律 中国兽医学报 2016,36 (2) 320-325
Position: Home > Articles > Tissue expression profile and expression pattern of Myf5 Gene in jinghai yellow chicken Chinese Journal of Veterinary Science 2016,36 (2) 320-325

京海黄鸡Myf5基因表达谱及表达规律

作  者：
张涛;张向前;董新龙;张跟喜;王金玉;樊庆灿;王文浩;王永娟
单  位：
扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室;江苏京海集团
关键词：
Myf5;京海黄鸡;表达谱;表达规律
摘  要：
为了探究Myf5基因的表达谱和表达规律,研究其可能的作用机理,本研究根据GenBank上的原鸡(Gallus gallus)Myf5基因cDNA序列,跨内含子设计1对荧光定量引物,采用实时荧光定量PCR技术检测Myf5基因在不同组织的表达情况,绘制其组织表达谱;并选取4个时间点,检测在高表达组织中的表达情况,以研究其表达规律。结果显示,无论是在公鸡还是母鸡中,Myf5基因均在胸肌和腿肌中的表达量最高。在公鸡的其他组织中表达量很低或几乎不表达,母鸡中,除在卵巢中有少量表达外,其他组织中表达量很低或几乎不表达。无论是在公鸡腿肌和胸肌中,还是在母鸡腿肌和胸肌中,Myf5基因的表达量总体呈下降趋势,在16周龄接近体成熟时表达量最低。本研究揭示了京海黄鸡(Gallus gallus)Myf5基因的表达谱以及随肌肉生长发育的表达规律,为进一步研究Myf5基因的作用机理、表达调控以及与MyoG基因相互作用机理奠定基础。
译  名：
Tissue expression profile and expression pattern of Myf5 Gene in jinghai yellow chicken
作  者：
ZHANG Tao;ZHANG Xiang-qian;DONG Xin-long;ZHANG Gen-xi;WANG Jin-yu;FAN Qing-can;Wang Wen-hao;WANG Yong-juan;Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province,College of Animal Science and Technology,Yangzhou University;Jiangsu Jinghai Group;
关键词：
Myf5;;Jinghai yellow chicken;;expression profile;;expression pattern
摘  要：
To explore the expression profile and expression pattern of Myf5 gene and its possible mechanism of action,apair of fluorescence quantitative primers was designed according to the cDNA sequence of Myf5 gene of Gallus gallus.Real-time fluorescent quantitative PCR was used to detect the expression level of Myf5 gene in different tissues to draw the expression profile.Then,the expression level of Myf5 gene in high expression tissue at four timing was detected to study the expression pattern.The results showed that Myf5 gene achieved the highest expression level in breast and leg muscle of male and female chicken.For male chicken,expression of Myf5 gene in other tissues except muscles was very low and barely detectable.For female chicken,little expression was detected in ovary and expression level in other tissues was barely detectable.The expression pattern of Myf5 gene showed a sustained downward trend in breast and leg muscle of male and female chicken.The Expression achieved the lowest level at 16 weeks of age,when the chicken reached body maturation.The present study revealed the expression profile and expression pattern with the growth and development of muscle of Jinghai yellow chicken(Gallus gallus).And it laided a foundation for further study on mechanism of action,expression regulation of MyoG gene and interaction between Myf5 and MyoG gene.

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