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Position: Home > Articles > Extraction and purification of polyphenol oxidase from litchi pulp Science and Technology of Food Industry 2010 (7) 203-205

荔枝果肉多酚氧化酶提取与初步纯化

作  者:
刘春丽;肖更生;刘亮;徐玉娟
单  位:
华南农业大学食品学院;广东省农业科学院蚕业与农产品加工研究所
关键词:
荔枝果肉;多酚氧化酶;提取;纯化
摘  要:
酶促褐变是影响荔枝果肉加工和贮藏的重要问题,多酚氧化酶(PPO)是引起酶促褐变的重要酶类。用磷酸缓冲液匀浆提取荔枝果肉中的多酚氧化酶,提取液最佳离子浓度为0·1mol/L、最适pH为7·5、最佳提取时间为2h,最适料液比为1∶3。并通过硫酸铵沉淀和Phenyl Sepharose CL-4B(苯基琼脂糖CL-4B)疏水柱层析进行初步纯化,该酶被纯化了9·4倍,得率为48%。
译  名:
Extraction and purification of polyphenol oxidase from litchi pulp
作  者:
LIU Chun-li1,2,3,4,XIAO Geng-sheng1,*,LIU Liang2,3,XU Yu-juan2,3 (1.College of Food Science,South China Agricultural University,Guangzhou 510642,China; 2.Sericulture & Fam Product Processing Research Institute,Guangdong Academy of Agricultural Science,Guangdong Open Access Laboratory of Product Processing,Guangzhou 510610,China; 3.Bio-tech Research Institute,Guangdong Academy of Agricultural Science,Guangzhou 510610,China; 4. Sichuan University of Science and Engineering,Zigong 643000,China)
关键词:
litchi pulp;polyphenol oxidase;extraction;purification
摘  要:
Enzyme browning is one of the most reactions impacting quality of litchi pulp. Polyphenol oxidase is a key enzyme inducing enzyme browning. Polyphenol oxidase in litchi pulp was extracted by phosphate buffer and purified by ammonium sulfate precipitation and Phenyl Sepharose CL-4B hydrophobic chromatography. Highest enzyme activities were obtained in a 0.1mol/L phosphate buffer at pH 7.5,the optimum extracting time was 2h and 1∶3 of the best solid to liquid. PPO from litchi pulp was purified by 9.4 fold and yield was 48%.

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