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当前位置: 首页 > 文章 > 曲酸遗传毒性的研究 食品科学 2011,32 (3) 228-232
Position: Home > Articles > Genetic Toxicity of Kojic Acid:Its Effects on Mice Mironucleus and DNA Damage in CHO-K1 Cells FOOD SCIENCE 2011,32 (3) 228-232

曲酸遗传毒性的研究

作  者：
陈永红;黄夏宁;邹志飞;谷康定;王岚;刘慧智
单  位：
广西大学轻工与食品工程学院;广东检验检疫技术中心;华中科技大学同济医学院公共卫生学院
关键词：
曲酸;传代细胞株;微核实验;单细胞凝胶电泳
摘  要：
目的:研究曲酸遗传毒性及有关作用机制。方法:采用小鼠骨髓细胞微核实验检测染色体损伤。用不同质量浓度曲酸(0、500、1000、1500、2000、2500μg/mL)诱导CHO-K1细胞,单细胞凝胶电泳实验检测CHO-K1细胞DNA损伤。结果:1/2LD50、1/4LD50和1/8LD50不同剂量曲酸诱导昆明小鼠微核实验结果显示,各质量浓度受试组与阴性对照组差别无显著性。单细胞凝胶电泳结果显示:曲酸作用质量浓度为1000μg/mL,作用时间为6h时,即出现轻微DNA损伤;当曲酸作用质量浓度增大到2500μg/mL时,出现明显的DNA损伤。在24h内,或其他同一作用时间段,随着曲酸作用质量浓度增加,DNA损伤也呈增加趋势。实验未发现有明显的时间-效应关系。结论:体内小鼠骨髓细胞微核实验未观察到曲酸明显的遗传毒性,但体外实验高质量浓度曲酸在一定时间可导致DNA损伤。
译  名：
Genetic Toxicity of Kojic Acid:Its Effects on Mice Mironucleus and DNA Damage in CHO-K1 Cells
作  者：
CHEN Yong-hong1,HUANG Xia-ning2,ZOU Zhi-fei1,GU Kang-ding2,WANG Lan1,LIU Hui-zhi1(1. Guangdong Inspection and Quarantine Technology Center,Guangzhou 510623,China;2. School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)
关键词：
kojic acid;cell line;micronucleus test;comet assay
摘  要：
Objective:To investigate the genetic toxicity and its mechanisms of kojic acid. Methods:Bone marrow micronucleus test in mice and comet assay in CHO-K1 cells were used to examine DNA damage. KM mice were randomly divided into 5 groups for 5 males and 5 females in each group. The KM mice were administered with kojic acid at doses of 1/2,1/4,and 1/8 fold LD50 for examining DNA damage. CHO-K1 cells were treated with kojic acid at the concentrations of 0,500,1000,1500,2000 μg/mL and 2500μg/mL for comet assay at 6,12,24 h and 48 h,respectively. Results:After treatment with kojic acid,the ratio of micronucleus in mice did not exhibit a significant increase,compared with the negative control. The slight DNA damage in CHO-K1 cells was observed in kojic acid group at the concentration of at 1000μg/mL after 6 h incubation. Severely DNA damage was also observed when kojic acid concentration was increased to 2500μg/mL. However,no response-time relationship was observed. Conclusion:Kojic acid at the experimental conditions did not result in genetic toxicity in bone marrow cells of treated mice,but could induce DNA damage in vitro at high concentration level.

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