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Position: Home > Articles > Embryogenic Callus Induction of Tall Fescue and Its Plant Regeneration Animal Husbandry and Feed Science 2009,30 (1) 65-67

高羊茅胚性愈伤组织诱导及植株再生

作  者:
Lan L
关键词:
ms;embryogenic callus induction;tall fescue;yemingzhu;xinxiu;rootin
摘  要:
对高羊茅2个品种(新秀和夜明珠2号)的再生技术进行了研究,建立了高频再生体系,为遗传转化奠定了基础。该试验以成熟的种子为外植体,诱导胚性愈伤组织。结果表明,2品种胚性愈伤组织诱导的最佳培养基为MS+2,4-D5.0mg/L+6-BA0.1mg/L;愈伤组织分化的最佳培养基:新秀为MS+6-BA2.0mg/L,夜明珠2号为MS+6-BA1.0mg/L;生根最佳培养基为MS+NAA0.5mg/L。
译  名:
Embryogenic Callus Induction of Tall Fescue and Its Plant Regeneration
作  者:
LI Lan,et al (College of Horticulture and Landscape, Southwest University, Beibei 400716,China)
关键词:
tall fescue; embryogenic callus; regeneration system
摘  要:
The regeneration technology of 2 tall fescue varieties (Xinxiu and Yemingzhu No. 2) was studied, the high frequency regeneration system was set up and foundation for genetic transformation was laid. With mature seeds as explant, embryogenic callus was induced. The best culture medium for embryogenic callus induction of 2 varieties was MS+2, 4-D 5.0 mg/L+6-BA 0.1 mg/L. The optimal medium for callus differentiation was MS+6-BA 2.0 mg/L for Xinxiu and MS+6-BA 1.0 mg/L for Yemingzhu No. 2, and the optimum medium for rooting was MS+NAA 0.5 mg/L.

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