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Position: Home > Articles > Screening and Identification of a Pseudomonas lutea Strain Capable of Degrading Caffeine FOOD SCIENCE 2010,31 (21) 218-221

可降解咖啡碱菌株的筛选与鉴定

作  者:
栗娜;籍保平;李博;吴薇
单  位:
中国农业大学食品科学与营养工程学院;中国农业大学工学院
关键词:
咖啡碱;假单胞菌;筛选;鉴定
摘  要:
目的:筛选能够降解咖啡碱的细菌,以期在该毒素的生物脱毒中得到应用。方法:以咖啡碱作为唯一碳源和氮源进行咖啡碱降解菌株的初筛,之后将初筛的10株菌通过液体培养基咖啡碱降解实验最终筛选出降解能力最强的1株菌。结果:筛选出的HZ-1菌株在48h即可将咖啡碱完全降解,对目标菌株细胞形态、生理生化以及16SrDNA进行鉴定,确定该菌株为Pseudomonas lutea,属于假单胞菌属。结论:利用咖啡碱作为唯一碳源和氮源从土壤中筛选出降解咖啡碱良好的菌株,为脱除咖啡碱提供了微生物菌种,填补了国内空白。
译  名:
Screening and Identification of a Pseudomonas lutea Strain Capable of Degrading Caffeine
作  者:
LI Na1,JI Bao-ping1,LI Bo1,WU Wei2(1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China ;2.College of Engineering,China Agricultural University,Beijing 100083,China)
关键词:
caffeine;Pseudomonas lutea;screening;identification
摘  要:
Objective:The aim of this work was to find strains capable of degrading caffeine so as to develop a biological approach for caffeine detoxicification.Method:The preliminary screening of target strains was performed using caffeine as both carbon and nitrogen sources,and then the ten screened strains were further screened by evaluating their abilities to degrade caffeine in liquid medium after culture.As a result,a strain with the strongest ability to degrade caffeine was obtained and named HZ-1.After 48 h culture of HZ-1,caffeine in liquid medium was completely degraded.Based on the morphological,physiological and biochemical properties and 16 rDNA gene sequence,this strain was identified as Pseudomonas lutea.To the best of our knowledge,this is the first report on the biological degradation of caffeine at home.

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