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Position: Home > Articles > Development of an efficient method for extracting total DNA of intestinal microflora. Animal Husbandry and Feed Science 2011,3 (3)

Development of an efficient method for extracting total DNA of intestinal microflora.

作者：

Yanhu Shen;Peng Zhang;Ding TieLin;Yang QuanQuan;Yan BingBing;Dongxu Su

单位：

School of Life Science, Xuzhou Normal University, Xuzhou 221116, China;School of Life Science, Xuzhou Normal University, Xuzhou 221116, Chin

关键词：

method;developed;result;stool;dna extraction method;qiaam

摘要：

[Objective] The aim was to develop a fast and effective DNA extraction method of intestinal microflora, a modified method of chloroform extraction, and to provide the basis for quantitative and qualitative detection. [Method] Through the improvement of conventional DNA extraction method, a rapid and efficient DNA extraction method was developed. Compared with the real-time PCR result of control sample and the result of QIAamp DNA Stool Mini kit, the developed method was verified. [Result] The DNA yield of the developed method was 100 times as much as that of QIAamp DNA Stool Mini kit. And the real-time PCR result showed that the efficiency of DNA extraction of the developed method was higher than that of the QIAamp DNA Stool Mini kit. [Conclusion] This modified method is inexpensive, efficient and rapid, and it is suitable for large quantities of feces samples.

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Development of an efficient method for extracting total DNA of intestinal microflora.

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