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当前位置: 首页 > 文章 > RP-HPLC法测定术苦芩颗粒中黄芩苷和苦参碱含量 中国兽医杂志 2015,51 (1) 97-100
Position: Home > Articles > Determination of Content of Baicalin and Matrine in Zhukuqin Granules by RP-HPLC Chinese Journal of Veterinary Medicine 2015,51 (1) 97-100

RP-HPLC法测定术苦芩颗粒中黄芩苷和苦参碱含量

作  者：
邱洪;朱兆荣;刘娟;凌榕镔;罗艺晨
单  位：
西南大学荣昌校区动物医学系
关键词：
反相高效液相色谱法;术苦芩颗粒;黄芩苷;苦参碱
摘  要：
为了建立测定术苦芩颗粒中黄芩苷、苦参碱含量的反相高效液相色谱法,采用Wonda Sil?C18柱(4.6 mm×250 mm,5μm)色谱柱,黄芩苷以甲醇-0.4%磷酸溶液(用三乙胺调节p H值至3.0)(52∶48,V/V)为流动相,检测波长为274 nm。苦参碱以乙腈∶甲醇∶0.2%磷酸溶液(用三乙胺调节p H值至7.0)(22∶13∶65,V/V)为流动相,流速为1.0 m L/min,检测波长220 nm;柱温30℃。术苦芩颗粒中黄芩苷和苦参碱分别在浓度为10μg/m L~150μg/m L和10μg/m L~400μg/m L范围内与峰面积均呈良好线性关系,r=0.99991和r=0.99994,平均加样回收率分别为99.32%和98.37%,其相对标准偏差(RSD)分别为0.625%和1.206%。用上述方法分别测定黄芩苷和苦参碱含量条件简单、稳定、快速,可用于检测术苦芩颗粒的质量控制标准。
译  名：
Determination of Content of Baicalin and Matrine in Zhukuqin Granules by RP-HPLC
作  者：
QIU Hong;ZHU Zhao-rong;LIU Juan;LING Rong-bing;LUO Yi-chen;Department of Veterinary Medicine,Southwest University;
关键词：
RP-HPLC;;Zhukuqin granules;;baicalin;;matrine
摘  要：
In order to establish a method for the determination of baicalin and matrine content in Zhukuqin granules. Chromatography was performed on a Wonda Sil?C18 column(4.6 mm×250 mm,5 μm),and acetonitrile-methanol-0.2% phosphoric acid solution(V:V =52∶48)as the mobile phase of baicalin was used at the wavelength of 274 nm and acetonitrile-methanol-0.2% phosphoric acid solution(V:V:V=22∶13∶65)as the mobile phase of matrine was used at the wavelength of 220 nm with 10 μL of the injection volume,at the flow rate of 1.0 m L/m L and column temperature of 30 ℃.The results showed that the calibration curve of baicalin was linear in the rang of 10μg/m L ~150 μg/m L(r=0.99991),and the average recovery rate was 99.32%(RSD=0.625%). And the calibration curve of matrine was linear in the rang of 10 μg/m L ~400 μg/m L(r=0.99994),and the average recovery rate was 98.37%(RSD=1.206%). The method was feasible and stable for the determination of baicalin and matrine content in Zhu Kuqin granules as the quality control standard.

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