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Position: Home > Articles > Determination of 2-Keto-D-gluconic Acid in Fermentation Broth by Polarimetry FOOD SCIENCE 2010,31 (22) 375-377

旋光法测定发酵液中2-酮基-D-葡萄糖酸

作  者:
孙文敬;周延政;冯晓燕;魏转;余泗莲;周强
单  位:
百勤异VC钠有限公司;河北师范大学生命科学学院;江苏大学食品与生物工程学院;河北化工医药职业技术学院制药工程系
关键词:
2-酮基-D-葡萄糖酸;葡萄糖;发酵液;旋光法;测定
摘  要:
通过测定发酵液的旋光度及其葡萄糖质量浓度,计算发酵液中2-酮基-D-葡萄糖酸的质量浓度,并考察该方法实际应用的可行性。结果表明:对照品溶液的旋光度、2-酮基-D-葡萄糖酸质量浓度和葡萄糖质量浓度之间存在良好的线性关系,R2>0.9999;该方法精密度、稳定性和重现性良好,2-酮基-D-葡萄糖酸的平均回收率为102.60%,RSD为2.17%(n=5)。该方法可用于发酵液中2-酮基-D-葡萄糖酸的定量检测。
译  名:
Determination of 2-Keto-D-gluconic Acid in Fermentation Broth by Polarimetry
作  者:
SUN Wen-jing1,ZHOU Yan-zheng1,FENG Xiao-yan2,WEI Zhuan3,YU Si-lian4,ZHOU Qiang4 (1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China ; 2. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China ; 3. Department of Pharmaceutical, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China ; 4. Parchn Sodium Isovitamin C Co. Ltd., Dexing 334221, China)
关键词:
2-keto-D-gluconic acid;glucose;fermentation broth;polarimetry;determination
摘  要:
The 2-keto-D-gluconic acid in fermentation broth was calculated on the basis of glucose concentration determined by optical rotation degree. The application feasibility of polarimetry was also investigated. Results indicated that a good linear relationship between optical rotation degree and the concentration of 2-keto-D-gluconic acid and glucose (R2 > 0.9999). The average recovery rate was 102.60% with RSD of 2.17%. This method has characteristics of precision, stability and reproducibility. Therefore, it can be used for determining 2-keto-D-gluconic acid in fermentation broth.

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