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Position: Home > Articles > Iodometric Determination of 2-Keto-D-gluconic Acid in Fermentation Broth FOOD SCIENCE 2010,31 (24) 314-317

碘量法测定发酵液中2-酮基-D-葡萄糖酸

作  者:
冯晓燕;周延政;孙文敬;王大明;余泗莲;刘敬泽
单  位:
百勤异VC钠有限公司;河北师范大学生命科学学院;江苏大学食品与生物工程学院
关键词:
2-酮基-D-葡萄糖酸;葡萄糖;发酵液;碘量法
摘  要:
在优化样品处理条件的基础上,考察葡萄糖对2-酮基-D-葡萄糖酸测定的影响,改进发酵液中2-酮基-D-葡萄糖酸的碘量法测定技术。结果表明:滴定样品溶液所消耗的I2标准溶液的体积、样品溶液中2-酮基-D-葡萄糖酸质量浓度和葡萄糖质量浓度之间存在良好的线性关系,R2>0.9999;该方法精密度、稳定性和重现性良好,2-酮基-D-葡萄糖酸的平均回收率为95.68%,RSD为1.35%(n=5)。该方法准确、快速,操作简便,可用于发酵液中2-酮基-D-葡萄糖酸的定量检测。
译  名:
Iodometric Determination of 2-Keto-D-gluconic Acid in Fermentation Broth
作  者:
FENG Xiao-yan1,ZHOU Yan-zheng2,SUN Wen-jing1,2,WANG Da-ming1,YU Si-lian3,LIU Jing-ze1, (1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;3. Parchn Sodium Isovitamin C Co. Ltd., Dexing 334221, China)
关键词:
2-keto-D-gluconic acid;glucose;fermentation broth;iodometry
摘  要:
Iodometric method was improved to prevent the interference of glucose in determination of 2-keto-D-gluconic acid (2KGA) in fermentation broth. The results revealed a good linear relationship among the volumes of I2 standard solution consumed and concentrations of 2-keto-D-gluconic acid and glucose (R2>0.9999). The average recovery was 95.68% with the relative standard deviation (RSD) of 1.35% (n=5). The method developed here had good accuracy, stability and reproducibility, and was easily operated, hereby could be used for the determination of concentrations of 2-keto-D-gluconic acid in fermentation broth.

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