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当前位置: 首页 > 文章 > 亲水作用色谱-串联质谱同时测定原料乳和液体乳中三聚氰胺和舒巴坦 食品工业科技 2017 (15) 244-247+252
Position: Home > Articles > Determination of melamine and sulbactam in raw milk and liquid milk by hydrophilic interaction chromatography tandem mass spectrometry Science and Technology of Food Industry 2017 (15) 244-247+252

亲水作用色谱-串联质谱同时测定原料乳和液体乳中三聚氰胺和舒巴坦

作  者：
薛霞;郑红;魏莉莉;刘艳明;祝建华;王骏
单  位：
山东省食品药品检验研究院
关键词：
三聚氰胺;舒巴坦;亲水作用色谱-串联质谱法;原料乳;液体乳
摘  要：
建立了亲水作用色谱-串联质谱同时测定原料乳和液体乳中三聚氰胺和舒巴坦的分析方法。试样采用2%乙酸水溶液提取,经乙腈沉淀蛋白,以乙腈-乙酸铵溶液为流动相,经Acquity UPLC BEH HILIC色谱柱分离后,采用多反应监测(MRM)扫描,外标法定量。三聚氰胺定量下限为10μg/kg,加标回收率为85.4%~102.5%,相对标准偏差为3.10%~6.55%。舒巴坦检定量下限为1.0μg/kg,加标回收率为86.1%~104.3%,相对标准偏差为2.35%~5.37%。该方法前处理简便快捷,灵敏度高,回收率和重现性良好,适用于原料乳和液体乳中三聚氰胺和舒巴坦的测定。
译  名：
Determination of melamine and sulbactam in raw milk and liquid milk by hydrophilic interaction chromatography tandem mass spectrometry
作  者：
XUE Xia;ZHENG Hong;WEI Li-li;LIU Yan-ming;ZHU Jian-hua;WANG Jun;Shandong Institute for Food and Drug Control;
关键词：
melamine;;sulbactam;;hydrophilic interaction chromatography tandem mass spectrometry(HILIC-MS/MS);;raw milk;;liquid milk
摘  要：
An analytical method was developed for the determination of melamine and sulbactam in raw milk and liquid milk using hydrophilic interaction chromatography tandem mass spectrometry( HILIC-MS/MS).The samples were extracted with 2%acetic acid solution,and the protein was precipitated with acetonitrile subsequently.The separations of melamine and sulbactam were carried out on an Acquity UPLC BEH HILIC column using ammonium acetate solution-acetonitrile as mobile phase.The target compounds were carried out in the multiple reaction monitoring( MRM) mode by employing the external standard method.The quantitation limit of mlamine was10 μg/kg.The recovery of melamine was 85.4% ~102.5%,with the RSD of 3.10%~6.55%.The quantitation limit of sulbactam was 1.0 μg/kg.The recovery of sulbactam was 86.1% ~104.3%,with the RSD of2.35% ~5.37%.With the advantages of convenience,sensitivity,good recoveries and repeatability,the method was suitable for the detection of melamine and sulbactam in raw milk and liquid milk.

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