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当前位置: 首页 > 文章 > 反相高效液相色谱法对人血浆中甲氨蝶呤的测定 湖北农业科学 2013,52 (24) 203-205
Position: Home > Articles > Determination of Methotrexate in Human Plasma with RP-HPLC Hubei Agricultural Sciences 2013,52 (24) 203-205

反相高效液相色谱法对人血浆中甲氨蝶呤的测定

作  者：
廖建华;余磊;李银保;李青松;张赛男
单  位：
赣南医学院科研中心;赣南医学院药学院
关键词：
反相高效液相色谱法;甲氨蝶呤;血药浓度
摘  要：
建立反相高效液相色谱法测定甲氨蝶呤血药浓度的方法,以指导临床合理用药。采用Agilent ZOBAX SB-C18柱(150 mm×4.6 mm,5μm);流动相为0.025 mol/L磷酸二氢钠溶液(pH 6.30)-甲醇(76/24,V/V);流速为1.00 mL/min;检测波长为313 nm;柱温为35℃;10%(V/V)高氯酸为蛋白沉淀剂。结果表明,血浆中甲氨蝶呤浓度在0.05~5.00μg/mL(Y1=35.556 C+0.771,r=0.999 9)和5.00~100.00μg/mL(Y2=36.727 C+1.700,r=0.999 9)范围内与峰面积呈良好线性关系,平均回收率为99.03%和97.04%。日内精密度小于1%,日间精密度小于2.5%(n=6),定量浓度为0.05μg/mL。该方法色谱条件稳定、操作简单、出峰时间快、线性范围宽、精密、准确、特异性强、回收率高,适用于甲氨蝶呤血药浓度的监测。
译  名：
Determination of Methotrexate in Human Plasma with RP-HPLC
作  者：
LIAO Jian-hua;YU Lei;LI Yin-bao;LI Qing-song;ZHANG Sai-nan;College of Pharmacy,Gannan Medical University;Research Center,Gannan Medical University;
关键词：
RP-HPLC;;methotrexate;;blood concentration
摘  要：
A RP-HPLC method for detecting methotrexate(MTX) in human plasma was established. The sample was separat- ed by Agilent ZOBAX SB-C18(150 mm×4.6 mm, 5 μm) column with mobile phase of sodium acetate buffer(pH 6.30)- methanol(76 / 24,V / V) at a flow rate of 1.0 mL / min. The wavelength of detection was 313 nm and the column temperature was 35 ℃. The plasma sample was treated with 10% perchloric acid for sedimentation. The results showed that the good lin- earity within 0.05~5.00 μg / mL(Y1 =35.556 C+0.771,r=0.999 9)and 5.00~100.00 μg / mL(Y2 =36.727 C+1.700,r =0.999 9)were observed. The average recovery rates were 99.03% and 97.04%, respectively. The RSD with intra-day was less than 1% and the inter-day was less than 2.5%(n=6). The LOQ was 0.05 μg / mL. The established method was simple, accurate, sensitive and reproducible with large-scale of detection range, suitable for monitoring the blood concentration of methotrexate.

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