中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 草鱼肌肉中呋喃唑酮代谢物AOZ检测能力验证样品的制备研究淡水渔业 2018 (5) 3-10

Position: Home > Articles > Preparation and study on the proficiency testing samples of furazolidone metabolite AOZ residual in Grass carp Freshwater Fisheries 2018 (5) 3-10

草鱼肌肉中呋喃唑酮代谢物AOZ检测能力验证样品的制备研究

作者：

田娟娟;甘金华;彭婕;李晋成;刘欢

单位：

上海海洋大学水产与生命学院;中国水产科学研究院;农业部水产品质量安全控制重点实验室;中国水产科学研究院长江水产研究所

关键词：

呋喃唑酮代谢物;检测;能力验证;样品制备;草鱼

摘要：

为解决现行水产品药物残留检测能力验证样品制备方式单一,现用能力验证样品实际检测分析难度与真实样品之间存在差距的现实问题,实验采用药浴给药的方式,研究了水温、药浴时间等因素对草鱼肌肉中AOZ残留浓度的影响。结果显示:在水温25℃、鱼体规格900~1 100 g、药浴10 h的条件下,草鱼肌肉中AOZ含量变化相对平稳,确立的药浴初始浓度x(μg/L)与草鱼肌肉中残留的AOZ浓度y(μg/kg)之间简单关式为y=0.22x。在此条件下制备了AOZ残留浓度为10.0μg/kg的能力验证样品,采用液相色谱-串联质谱法对样品的均匀性和稳定性进行了评价。样品检测结果均匀性和稳定性良好,符合能力验证样品的要求。

译名：

Preparation and study on the proficiency testing samples of furazolidone metabolite AOZ residual in Grass carp

作者：

TIAN Juan-juan;GAN Jin-hua;PENG Jie;LI Jin-cheng;LIU Huan;College of Fisheries and Life Science,Shanghai Ocean University;Chinese Academy of Fishery Sciences;Key Labrotary of Control of Quality and Safety for Aquatic Products,Minisrty of Agriculture;Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences;

关键词：

furazolidone metabolites;;detection;;proficiency testing;;sample preparation;;Grass carp

摘要：

The effects of water temperature,bath time and other factors on the residual concentration of furazolidone metabolites in Grass carp muscle were studied by means of drug bath administration to solve the problem of the sample preparation method is simple of proficiency testing. Meanwhile,the practical detection and analysis difficulty problem of the gap between the current proficiency testing samples and the real samples were also research objectives.. The results showed that the change of AOZ content in the muscle of the grass carp was relatively stable under the conditions of water temperature 25℃,fish size ranged from 900 to 1100 g and drug bath time 10 h. The relationship between initial concentration of drug bath x( μg/L) and the residual AOZ in muscle concentration y( μg/kg) is y = 0. 22 x,The homogeneity and stability of the prepared sample were detected by liquid chromatography-tandem mass spectrometry. The sample has a good homogeneity,stability and meets the requirements of proficiency testing.

相似文章

酶联免疫法检测猪肉中呋喃唑酮代谢物残留 [闵成军, 罗晓琴, 汪善良, 崔廷婷] 肉类研究 2011 (12) 29-32
呋喃唑酮代谢物人工抗原的合成及半抗原偶联比的测定 [曾磊, 周常义, 苏国成] 安徽农业科学 2013,41 (3) 33-34+94
酶联免疫法测定水产品中呋喃唑酮代谢物残留量 [葛怀礼, 蒙君丽, 宋荪阳, 李爱军, 董李学] 河北渔业 2014 (8) 25-27+32
呋喃唑酮代谢物在食品检测中的研究新进展 [徐骏, 刘坤, 李丹, 陈晨, 任峥峰, 张文] 吉林农业 2019 (17) 86
化学发光免疫分析与酶联免疫分析法检测水产品药物残留的比较研究 [萨仁托雅, 张峰, 郑有虎, 卢亚楠] 大连海洋大学学报 2014 (5) 66-71
硝基呋喃类代谢物抗原合成及在胶体金试纸条上的应用 [柳爱春, 刘超, 桑丽雅, 王振国] 现代农业科技 2012 (23) 267-269+275
呋喃唑酮代谢物人工抗原的合成及抗体的制备 [贾慧勤, 丁焕中, 刘晓云, 李小红, 张炳旭, 鲁晓雄] 中国兽药杂志 2013,47 (6) 20-23
高效液相色谱-串联质谱法检测鸡蛋中呋喃唑酮代谢物的残留 [耿士伟, 朱永林, 邵德佳] 兽药与饲料添加剂 2005,10 (5)
酶联免疫法测定水产品中呋喃唑酮代谢物AOZ的残留 [沈美芳, 宋红波, 耿雪冰, 吴光红] 水产学报 2006,30 (4) 90-94
胶体金免疫层析法快速测定动物源食品中的呋喃唑酮代谢物残留 [潘嘉慧, 田峻, 沈国权, 赵亮亮, 朱海云] 兽医导刊 2014 (16) 25-26

导出引用

计量

文章访问数: 12

HTML全文浏览量: 0

PDF下载量: 0

草鱼肌肉中呋喃唑酮代谢物AOZ检测能力验证样品的制备研究

相似文章

所属期刊

淡水渔业

推荐期刊

江苏农业科学

中国渔业质量与标准