当前位置: 首页 > 文章 > 硝基呋喃类代谢物抗原合成及在胶体金试纸条上的应用 现代农业科技 2012 (23) 267-269+275
Position: Home > Articles > Synthesis of Antigen of Nitrofuran Metabolites and Application of Colloidal Gold Rapid Test Device Modern Agricultural Science and Technology 2012 (23) 267-269+275

硝基呋喃类代谢物抗原合成及在胶体金试纸条上的应用

作  者:
柳爱春;刘超;桑丽雅;王振国
单  位:
浙江省杭州市农业科学研究院;杭州南开日新生物技术有限公司
关键词:
呋喃唑酮代谢物;免疫胶体金;人工抗原;快速检测
摘  要:
在酸性条件下,以4-羰基苯甲醛为衍生化试剂分别与4种硝基呋喃类代谢物(AOZ、SEM、AMOZ和AHD)反应制成相应半抗原:CPAOZ、CPSEM、CPAMOZ和CPAHD,采用碳二亚胺法将半抗原与牛血清蛋白偶联合成人工抗原:CPAOZ-BSA、CPSEM-BSA、CPAMOZ-BSA和CPAHD-BSA,产物经紫外扫描鉴定,表明抗原偶联牛血清蛋白成功。在此基础上研制成4种硝基呋喃代谢物快速胶体金试剂条,最低检出限分别为0.5、1.0、1.0、2.0μg/kg,相互间的交叉反应率小于0.1%。
译  名:
Synthesis of Antigen of Nitrofuran Metabolites and Application of Colloidal Gold Rapid Test Device
作  者:
LIU Ai-chun 1 LIU Chao 1 SANG Li-ya 2 WANG Zhen-guo 2(1 Hangzhou Academy of Agricultural Sciences in Zhejiang Province,Hangzhou Zhejiang 310024;2 Hangzhou Nankai Biotech Co.,Ltd.)
关键词:
metabolite of nitrofurans;colloidal gold;artificial antigen;rapid test
摘  要:
With 4-carbonyl benzaldehyde for derivatization reagent,four nitrofuran metabolites(AOZ,SEM,AMOZ and AHD)were made into the corresponding haptens:CPAOZ,CPSEM,CPAMOZ and CPAHD in the acidic conditions,and the haptens were successfully conjugated to bovine serum albumin(BSA)with the carbodiimide method to form artificial antigens,such as CPAOZ-BSA,CPSEM-BSA,CPAMOZ-BSA and CPAHD-BSA.Products by UV scanning showed success of antigens linked into BSA.On the basis of this study,colloidal gold rapid test devices for nitrofuran metabolites were successfully developed.The minimum detection limits were 0.5 μg/kg,1.0 μg/kg,1.0 μg/kg and 2.0 μg/kg respectively,and mutual cross reaction rate was less than 0.1%.

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