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Position: Home > Articles > Optimization of the Double-enzymatic Extraction of Procyanidins from Lotus Seed Pods Using Response Surface Methodology FOOD SCIENCE 2011,32 (4) 64-68

响应曲面法优化双酶法提取莲房原花青素

作  者：
汪志慧;孙智达;谢笔钧
单  位：
华中农业大学食品科学技术学院
关键词：
莲房;原花青素;酶解;提取率;中心组合设计;响应面
摘  要：
利用Box-Behnken中心组合试验设计及响应面(RSM)分析,研究双酶法提取莲房原花青素(LSPC)的浸提条件对原花青素提取率的影响,利用SAS软件对莲房原花青素提取率的二次回归模型进行分析。结果表明:双酶法提取莲房原花青素的最佳工艺参数为酶解温度53℃、酶解时间1.6h、pH4.8、果胶酶:纤维素酶=1:1.1,在此工艺参数下原花青素的提取率为5.20%,优化后的工艺相对单一乙醇提取法提取率的3.84%,有明显的提高。采用DPPH法进行抗氧化性的对比,结果显示双酶法提取的莲房原花青素比单一醇法提取的原花青素的清除DPPH自由基的能力强。因此,由Box-Behnken响应曲面法优化所得的双酶法提取工艺可为莲房原花青素的工业提取提供参考和依据。
译  名：
Optimization of the Double-enzymatic Extraction of Procyanidins from Lotus Seed Pods Using Response Surface Methodology
作  者：
WANG Zhi-hui,SUN Zhi-da,XIE Bi-jun (College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)
关键词：
lotus seed pod;procyanidins;enzymatic hydrolysis;extraction rate;central composite design;response surface methodology
摘  要：
A process for extracting procyanidins from lotus seed pods consisting of simultaneous double-enzymatic hydrolysis with pectinase and cellulose and subsequent extraction with 65% aqueous ethanol solution was proposed in this study. Central composite design (CCD) coupled with response surface methodology (RSM) was used to investigate the optimization of the double-enzymatic hydrolysis of lotus seed pods for improved procyanidin extraction. A mathematical predictive model for the extraction yield of procyanidins was established based on the experimental data from CCD design,and analyzed using SAS software. The results showed that the optimal technological conditions for the hydrolysis of lotus seed pods were as follows:hydrolysis temperature,53 ℃; hydrolysis time,1.6 h; initial hydrolysis pH,4.8; and the ratio of pectinase to cellulose,1:1.1,and the yield of procyanidins was 5.20% after hydrolysis under these conditions followed by 120 min extraction at 55 ℃with 65% aqueous ethanol solution,35.7% higher than that of single ethanol extraction. In addition,procyanidins extracted by the method had stronger ability to scavenge DPPH radicals than those derived from single ethanol extraction.

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