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当前位置: 首页 > 文章 > 莲原花青素抗黑色素瘤的研究 食品科学 2009,30 (11) 223-226
Position: Home > Articles > in vivo and in vitro Inhibitory Activities of Proanthocyanidins from Lotus Seedpod (LSPC) and Lotus Seed Hulls (LSHPC) against Melanoma B16 in Mice FOOD SCIENCE 2009,30 (11) 223-226

莲原花青素抗黑色素瘤的研究

作  者：
周密;段玉清;王文兵;李金凤;徐菲菲;谢笔钧
单  位：
江苏大学生命科学研究院;江苏大学食品与生物工程学院;华中农业大学食品科技学院
关键词：
莲房;莲子壳;原花青素;黑色素瘤B16;抑制作用
摘  要：
目的:探讨莲房和莲子壳中原花青素对黑色素瘤B16生长的影响。方法:采用MTT法测定莲房和莲子壳原花青素对B16细胞的体外抑制作用;并借助显微技术观察B16细胞形态;以荷黑色素瘤的C57BL/6J小鼠为体内研究对象,通过瘤体积和瘤重测定,探讨体内抑瘤作用,采用黄嘌呤氧化酶法和硫代巴比妥酸法测定荷瘤鼠血清中超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果:莲房和莲子壳中原花青素体外对黑色素瘤B16细胞均有较强的抑制作用,抑制率分别为87.4%和78.2%,均使细胞的膜破损,细胞形态改变;体内能显著减小瘤体积和减轻瘤重,抑瘤率分别为55.3%和46.7%,并能有效降低荷瘤小鼠血清中MDA含量,提高SOD活力。结论:莲房和莲子壳中原花青素对黑色素瘤均具有较强的抑制作用。
译  名：
in vivo and in vitro Inhibitory Activities of Proanthocyanidins from Lotus Seedpod (LSPC) and Lotus Seed Hulls (LSHPC) against Melanoma B16 in Mice
作  者：
ZHOU Mi1,DUAN Yu-qing2,WANG Wen-bing1,LI Jin-feng2,XU Fei-fei2,XIE Bi-jun3 (1.Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China ; 2.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China ; 3.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
关键词：
lotus seedpod;lotus seed hulls;proanthocyanidins;melanoma B16;inhibitory effect
摘  要：
Proanthocyaidins were extracted from lotus seedpod (LSPC) and lotus seed hulls (LSHPC) with 1% NaHSO3 solution by boiling for 15 min and purified with AB-8 macroporous resin. After incubated with culture medium containing different concentrations of the proanthocyaidins from LSPC and LSHPC for 48 h and 72 h, the viability of mouse melanoma B16 cells was measured by MTT assay, and their morphology was observed under inverted microscope and fluorescence microscope after 72 h incubation with medium containing the two proanthocyaidins at 100 μ g/ml. The in vivo inhibitory effect against the proliferation of mouse melanoma B16 cells was investigated by using C57BL/6J mice, and the levels of superoxide dismutase (SOD) and malonicaldehyde (MDA) in mouse serum were determined by xanthine oxidase assay and thiobarbituric acid assay. Results showed that both the proanthocyaidins from LSPC and LSHPC were able to significantly inhibit proliferation of B16 cells in vitro, and the inhibition rates were 87.4% and 78.2%, respectively. The morphological alterations of melanoma B16 cell membrane damage caused by them were observed. Both the two proanthocyaidins could significantly reduce the volume and mass increases of melanoma B16 in in vivo experiments, and their inhibition rates at 120 mg/kg bw against the mass of melanoma B16 reached 55.3% and 46.7%, respectively. Serum MDA level was reduced by administration of the proanthocyaidins from LSPC and LSHPC accompanied with the enhancement in serum SOD activity.

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