当前位置: 首页 > 文章 > 不同粒径纳米金标记二抗增强表面等离子共振检测E.coli O 157:H7 食品科学 2015,36 (8) 201-205
Position: Home > Articles > Detection of E. coli O157:H7 Based on Au Nanoparticles of Different Sizes Labeled Second Antibody Enhanced Surface Plasmon Resonance FOOD SCIENCE 2015,36 (8) 201-205

不同粒径纳米金标记二抗增强表面等离子共振检测E.coli O 157:H7

作  者:
杨阳;李荣卓;毛禄刚;刘霞
单  位:
湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室;湖南农业大学食品科学技术学院
关键词:
金纳米粒子;E.coli O157∶H7;表面等离子共振传感器;标记;不同粒径
摘  要:
基于双通道表面等离子共振(surface plasmon resonance,SPR)传感器,结合不同粒径的金纳米粒子(Au nanoparticle,Au NPs)标记多克隆抗体(polyclonal antibody,PAb)作为第二抗体,采用氨基偶联的方法将PAb固定在传感器表面作为第一抗体,采用三明治夹心法进行了检测大肠杆菌O157∶H7(E.coli O157∶H7)的研究。考察3种粒径的Au NPs作为标记物,增强SPR响应信号检测E.coli O157∶H7的能力。结果表明,增强效果最佳的Au NPs粒径为17.79 nm,其可检测到的E.coli O157∶H7的最低浓度为10 CFU/m L。
译  名:
Detection of E. coli O157:H7 Based on Au Nanoparticles of Different Sizes Labeled Second Antibody Enhanced Surface Plasmon Resonance
作  者:
YANG Yang;LI Rongzhuo;MAO Lugang;LIU Xia;Hunan Province Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology,Hunan Agricultural University;
关键词:
Au NPs;;E.coli O157:H7;;surface plasmon resonance(SPR);;labeling;;different particle sizes
摘  要:
Escherichia coli O157:H7(E. coli O157:H7) was detected by a sandwich method with Au nanoparticles(Au NPs) of different sizes labeled E. coli O157:H7 polyclonal antibody(PAb) as the second antibody and PAb immobilized on the sensor surface as the first antibody using a dual-channel surface plasmon resonance(SPR) sensor. The abilities of second antibodies labeled with Au NPs of three different sizes to enhance SPR signal were compared. As a result, the optimal Au NPs size was found to be 17.79 nm and the minimum detectable concentration of E. coli O157:H7 was 10 CFU/m L.

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