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Position: Home > Articles > Purification and Partial Characterisation of Feruloyl Esterase from Barley Malt China Animal Husbandry & Veterinary Medicine 2010,37 (1) 28-31

大麦芽阿魏酸酯酶的分离纯化及其部分酶学性质的测定

作  者:
刘莎莎;李华;石庆华;王希东;程发祥
单  位:
新疆农业大学农学院;中国农业大学;新疆农业大学动物科学学院动物营养实验室
关键词:
大麦芽;阿魏酸酯酶;分离纯化;酶学性质
摘  要:
本试验采用硫酸铵分级沉淀、透析、DEAE-52离子交换层析对大麦芽中的阿魏酸酯酶(FAE)进行了分离纯化,并测定了其部分酶学性质。结果显示,分离纯化后获得了阿魏酸酯酶纯品,纯化倍数达34倍;经SDS-PAGE电泳显示为单一条带,并测定其相对分子质量约为29.3 ku;最适反应温度为50℃;最适pH为5.5,但阿魏酸酯酶在60℃以下,pH 4.5~7.5之间有较好的稳定性;Zn2+、Cu2+、Fe3+对酶活有很强的抑制作用,Na+和EDTA对酶有一定的激活作用。
译  名:
Purification and Partial Characterisation of Feruloyl Esterase from Barley Malt
作  者:
LIU Sha-sha1,LI Hua2,SHI Qing-hua1,WANG Xi-dong1,CHENG Fa-xiang3(1.College of Agronomy Xinjiang Agricultural University,Urumqi 830052,China;2.China Agricultural University(Yantai),Yantai 264670,China;3.Laboratory of Animal Nutrition,College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China)
关键词:
barley malt;feruloyl esterase;isolation and purification;enzyme characterisation
摘  要:
The activity of feruloyl esterase has been enhanced in barley grain and barley malt.Feruloyl esterase solution was extracted from barley malt has been purified by ammonium sulfate precipitation,DEAE-cellulose column chromatography.The feruloyl esterase was purified 34-fold as shown in SDS-PAGE gel electrophoresis and the molecular weight estimated to be about 29.3 kD.Characterization of the enzyme exhibited that the optimum pH and the optimum temperature were 5.5 and 50 ℃,respectively,and it lost little activity after one hour below 60 ℃ from pH 4.5 to 7.5.It was inhibited by Zn2+,Fe3+,Cu2+,and strongly activated by EDTA and Na+.

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