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Position: Home > Articles > Homogenate extraction technology of camptothecine and hydroxycamptothecin from Camptotheca acuminata leaves. Journal of Forestry Research 2009,20 (2) 168-170

Homogenate extraction technology of camptothecine and hydroxycamptothecin from Camptotheca acuminata leaves.

作  者:
Shi WeiGuo;Zu YuanGang;Zhao ChunJian;Yang Lei
单  位:
China.; Harbin 150040;Key Laboratory of Forest Plant Ecology of Ministry of Education; Northeast Forestry University
关键词:
alkaloids;chemical composition;ethanol;extraction;leaves;plant composition;Camptotheca acuminata;China;Sichuan
摘  要:
Camptothecine (CPT) and hydroxycamptothecin (HCPT), two kinds of anti-cancer alkaloids, were extracted from Camptotheca acuminata leaves from Sichuan, China, using homogenate extraction technology under different conditions such as the ratio of material to liquid, ethanol concentration, and homogenate time. The optimum technology parameters for homogenate extraction of CPT and HCPT from C. acuminata leaves were determined as homogenate time at 8 minutes, ethanol concentration at 55% and the ratio of material to liquid at 1:15 (g:mL). By using the optimized parameters, we obtained 0.639 per mil extraction rate for CPT and 0.437 per mil for HCPT. The extraction yields of CPT and HCPT extracted by homogenating technology were higher than those by other extractive methods, such as ultrasonic, reflux, shaking in water bath. It is concluded that the homogenate extraction technology was an efficient method for extracting CPT and HCPT from C. acuminata leaves, with characteristics of less extraction time and high yield.

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