当前位置: 首页 > 文章 > 产S-腺苷甲硫氨酸菌株的筛选及发酵工艺优化 华中农业大学学报 2011,30 (1) 115-120
Position: Home > Articles > Screening of Saccharomyces cerevisiae producing S-adenosylmethionine and optimization of fermentation process Journal of Huazhong Agricultural University 2011,30 (1) 115-120

产S-腺苷甲硫氨酸菌株的筛选及发酵工艺优化

作  者:
万荣娥;王启明;张喜团;熊善柏
单  位:
中国科学院微生物研究所;华中农业大学食品科技学院
关键词:
酿酒酵母;S-腺苷甲硫氨酸;发酵工艺
摘  要:
以45株酵母菌株作为出发菌株,筛选出2株高产SAM(S-腺苷甲硫氨酸)的酿酒酵母菌株(A12A、A18A),以该2株酿酒酵母菌株为对象,研究发酵温度、发酵液初始pH值、摇床转速以及发酵时间对菌体产量和SAM产量的影响,并优化发酵工艺条件,以提高SAM的产量。结果表明:发酵温度、发酵液初始pH值、摇床转速和发酵时间对菌体产量和SAM产量均有显著影响。经正交试验优化,2株酿酒酵母菌株产SAM的最佳发酵条件为25℃、发酵液初始pH值5.0、摇床转速225 r/min、发酵时间108 h,在该发酵条件下,酿酒酵母菌株A18A产SAM最高量达2.54 g/L,较优化前提高1.17倍。
译  名:
Screening of Saccharomyces cerevisiae producing S-adenosylmethionine and optimization of fermentation process
作  者:
WAN Rong-e1 WANG Qi-ming2 ZHANG Xi-tuan1 XIONG Shan-bai11.Department of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070 China;2.Institute of Microbiology Chinese Academy of Sciences,Beijing 100101,China
关键词:
Saccharomyces cerevisiae;S-adenosyl-L-methionine;fermentation process
摘  要:
The effects of fermentation temperature,the initial pH of fermentation broth,shaking speed and feeding time on the cell dry weight and yield of S-adenosyl-L-methionine(SAM) produced by Saccharomyces cerevisiae(A12A,A18A) selected from 45 yeast strains were studied,and the fermentation conditions was optimized.The results showed that cell dry weight and SAM production were significantly affected(P<0.05) by fermentation temperature,the initial pH,rotation speed and the feeding time.With the orthogonal optimization,the most suitable conditions for SAM accumulation are temperature 25 ℃,initial pH 6.0,shaking speed 225 r/min,and feeding time 108 h.Under fermentation conditions mentioned above,the SAM production of Saccharomyces cerevisiae A18A was up to 2.54 g/L broth,1.17-fold higher than before optimization.

相似文章

计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊