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高通量生物分析技术及应用研究进展

作  者:
翟绪昭;王广彬;赵亮涛;曾海娟;李建武;丁承超;宋春美;刘箐
单  位:
上海理工大学医疗器械与食品学院;徐州绿健乳业有限责任公司;甘肃省兰州大学第二医院
关键词:
高通量;芯片;免疫分析技术;食品安全
摘  要:
基因组学、蛋白组学、代谢组学等研究的兴起使得大量生物数据的快速获取和分析变得更加重要。传统的生物分析方法大多耗时、费力,已无法满足现代生命科学研究对海量生物信息的需要。高通量分析技术是快速获取大量生物信息的重要手段。对微阵列芯片、微流控芯片、焦磷酸测序、荧光偏振免疫分析、量子点荧光免疫分析等高通量生物分析技术进行了综述,简述了近几年高通量生物分析技术的研究重点和研究成果,并对其在食品安全、医学等方面的应用进行了简要介绍。
译  名:
An Overview of High Throughput Biological Screening Methods and Its Application
作  者:
ZHAI Xu-zhao;WANG Guang-bin;ZHAO Liang-tao;ZENG Hai-juan;LI Jian-wu;DING Cheng-chao;SONG Chun-mei;LIU Qing;School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology;Xuzhou Lüjian Dairy Co.,Ltd;Laboratory of Medical Genetics,the Second Hospital of Lanzhou University;
关键词:
high throughput;;chip;;immunoassay;;food safety
摘  要:
The emergence of genomics,proteomics,metabolomics and other molecular biology studies makes the rapid attainment and assessment of large amounts of biological data become extremely important. The traditional detection methods are time-consuming and laborious,and cannot satisfy the needs of contemporary biological science research for massive biological information. High Throughput Screening(HTS)methods are significant means of quick attainment of massive biological information. This paper will make an overview of microarray chip,microfluidic chip,pyrosequencing,fluorescence polarization immunoassay,quantum dot fluorescence immunoassay and multiple PCR,outline research focus and research results of HTS methods in recent years,and briefly introduce its application in food safety,medicine and other fields.

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