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Position: Home > Articles > Study on the Preparation and Regeneration Condition of Rhodotorula Protoplast Journal of Anhui Agricultural Sciences 2010,38 (3) 1154-1155+1172

红酵母原生质体制备和再生条件研究

作  者：
詹萍;苏龙;周乃东
单  位：
玉林师范学院化学与生物系
关键词：
红酵母;原生质体制备;原生质体再生
摘  要：
[目的]初步探讨红酵母原生质体制备和再生的条件。[方法]以红酵母为试材,研究渗透压稳定剂pH值、酶浓度、酶解时间、预处理时间4个因素对红酵母原生质体制备率和再生率的影响。[结果]pH值在6.5~7.0,原生质体的制备率和再生率随着渗透压稳定剂pH值的升高而上升。当酶浓度从0.2%上升到1.0%时,原生质体制备率上升较快,而原生质体再生率下降较慢。随着酶解时间的增加,原生质体的制备率逐渐增加,而原生质体的再生率逐渐下降。在5~15 m in,原生质体制备率和再生率均随着预处理的时间的增加而降低。[结论]当渗透压稳定剂pH值为7.0,蜗牛酶酶浓度为1.0%,酶解时间为60 m in,预处理时间为5 m in时,原生质体的制备率和再生率能分别达到较高的值。
译  名：
Study on the Preparation and Regeneration Condition of Rhodotorula Protoplast
作  者：
ZHAN Ping et al(Department of Chemistry and Biology,Yulin Normal University,Yulin,Guangxi 537000)
关键词：
Rhodotorula;Protoplast preparation;Protoplast regeneration
摘  要：
[Objective] The aim was to preliminarily research the protoplast preparation and regeneration condition of rhodotorula.[Method] With the rhodotorula as the tested material,the effects of four factors including the pH value of osmotic stabilizer,enzyme concn.,enzymolysis time and pretreatment time on the preparation and regeneration rate of Rhodotorula protoplast were studied.[Result] The protoplast preparation rate and regeneration rate were raised with the rising of the pH value of osmotic stabilizer when the pH value was in the range of 6.5-7.0.The protoplast preparation rate was raised faster but the protoplast regeneration rate was descended slower when the enzyme concn.was raised from 0.2% to 1.0%.With the rising of the enzymolysis time,the protoplast preparation rate was increased gradually,but the protoplast regeneration rate was decreased gradually.The protoplast preparation rate and regeneration rate all were decreased with the rising of pretreatment time in the range of 5-15 min.[Conclusion] The protoplast preparation rate and regeneration rate were up to the higher value resp.when the pH value of osmotic stabilizer,the enzyme concn.of snail enzyme,enzymolysis time and pretreatment time was 7.0, 1.0%,60 min,5 min resp.

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