中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 响应面法优化制备高富硒产朊假丝酵母 农业资源与环境学报 2014 (06) 575-582
Position: Home > Articles > Optimization of Selenium-enriched Candida utilis by Response Surface Methodology Journal of Agricultural Resources and Environment 2014 (06) 575-582

响应面法优化制备高富硒产朊假丝酵母

作  者：
张帆;谢丽;邹晓阳;朱建航
单  位：
南昌大学环境与化学工程学院化工系;丰城市中国生态硒谷现代农业管委会
关键词：
产朊假丝酵母;富硒;响应面法;总硒含量
摘  要：
考察了发酵条件对产朊假丝酵母富硒能力的影响。通过单因素的筛选,对酵母富硒能力影响较大的3个因素:亚硒酸钠浓度、初始p H值及培养温度,以胞内总硒含量为响应值,利用响应面法对其进行优化。结果显示:在培养时间30 h、加硒时间对数生长中期、亚硒酸钠浓度35 mg·L-1,初始p H 6.6、接种量10%、培养温度27℃、装液量150 m L/500 m L的条件下,最大的菌体生物量为6.87 g·L-1;胞内总硒含量达到12 639.7μg·L-1,硒含量为1 839.8μg·g-1,其中亚硒酸钠转化率为79.1%,有机硒含量占90%以上;胞内实际总硒含量与数学模型理论值12 518.8μg·L-1相差不显著,响应面法能较好地优化产朊假丝酵母富硒工艺条件。
译  名：
Optimization of Selenium-enriched Candida utilis by Response Surface Methodology
作  者：
ZHANG Fan;XIE Li;ZOU Xiao-yang;ZHU Jian-hang;School of Environmental and Chemical Engineering, Nanchang University;Modern Ecological Agricultural Management Committee of Selenium-enriched Valley;
关键词：
Candida utilis;;selenium enriched;;response surface methodology;;total selenium content
摘  要：
The fermentation conditions of selenium enrichment by Candida utilis were studied. Based on the results of the single factor experiment, three factors including the concentration of sodium selenite, inital p H and incubation temperature were selected. The response surface method was used to optimize the various factors. The optimal conditions were obtained as follows: incubation time was 30 h, time of adding selenium was mid-logarithmic, the sodium selenite concentration was 35 mg·L-1with inital p H of 6.6, incubation concentration of 10%, incubation temperature of 27 ℃, the medium volume of 150 m L/500 m L, respectively. Under the optimal condition, the biomass was 6.87 g·L-1.The total selenium content of Candida utilis was 12 639.7 μg·L-1, and the selenium content of the cells was 1 839.8 μg·g-1, in which sodium selenite conversion rate was 79.1% and the organic selenium was higher than 90%. The actual value of selenium content was substantially consistent with the theoretical value, and the response surface methodology was applicable for the fermentation conditions of selenium enriched by Candida utilis.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46