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Position: Home > Articles > Determination of Platycodin Dof Radix Platycodiin Different Places by RP- HPLC Journal of Jilin Agricultural University 1999,21 (4) 35-38

应用RP-HPLC法测定不同产地中桔梗皂甙D

作  者:
许传莲;杨腊虎;郑毅男;刘墨祥;徐宝军
单  位:
吉林农业大学中药材学院;中国生物制品药品检定所
关键词:
RP-HPLC;桔梗;桔梗皂甙D
摘  要:
用反相高效液相色谱(RP- HPLC) 分析方法对常用中药桔梗中主要有效成分桔梗皂甙D(platycodinD) 进行了研究,考察了流动相对分离的影响,选择以C18 为色谱分析柱,乙腈- 磷酸盐缓冲液为流动相,检测波长为210 nm ,进样量在2-0 ~32-0 μg 之间呈良好线性关系,平均回收率为98-13 % ,RSD 为2-3 % 。
译  名:
Determination of Platycodin Dof Radix Platycodiin Different Places by RP- HPLC
作  者:
XU Chuan lian , YANGLa hu ,ZHENG Yi nan , LIU Mo xiang et al. ( College of Chinese Medicinal Material, Jilin Agricultural University ,Changchun 130118)
关键词:
RP HPLC; Platycodon ;platycodin D
摘  要:
This paper deals with a quantitative study on platycodin Dby RP- HPLC-The analyt icalcolumn used is Microsorb C18-The mobile phase was CH3CN- KH2PO4(0-05mol/L)(30  70) ,pH 3-1 ,regulated by H3PO4-Flow rate was 0-7 m L/min-The detection wavelength was 210 nm-The linear ranges of platycodin D was 2-0 ~32-0 μg-The accuracy of method was checked by recoveries-The recovery of platycodin D was 98-13 % -The analytical results showed that the content of platycodin Din samples showed great different between different areas-

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