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Position: Home > Articles > HPLC Determination of Formononetin in Corn Silk FOOD SCIENCE 2009,30 (6) 178-179

高效液相色谱法测定玉米须中刺芒柄花素的含量

作  者:
张彦青;鲁晓翔;董彩霞
单  位:
天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室
关键词:
玉米须;刺芒柄花素;高效液相色谱
摘  要:
目的:建立玉米须中刺芒柄花素含量测定的方法。方法:运用高效液相色谱法测定,色谱柱为Hypersil C18(4.6mm×250mm,5μm),流动相为甲醇-0.1%磷酸(55:45,V/V),流速0.8ml/min;检测波长254nm。结果:刺芒柄花素在2.3~23μg/ml范围内线性关系良好,r=0.9991。平均回收率为96.93%,RSD为1.30%。结论:本法方便、准确,可以用于玉米须的质量控制。
译  名:
HPLC Determination of Formononetin in Corn Silk
作  者:
ZHANG Yan-qing,LU Xiao-xiang,DONG Cai-xia (College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China)
关键词:
corn silk;formononetin;HPLC
摘  要:
Objective: To develop an HPLC method for the determination of formononetin in corn silk. Method: Analysis was carried out by using Hypersil C18 (4. 6 mm ×250 mm, 5 μm) column with CH3OH-0.1% H3PO4 (55:45, V/V) as the mobile phase. The flow rate was 0.8 ml/min and the UV detection wavelength was set at 254 nm. Results: There is good linear relationship between integral value (Y) of peak area and formononetin reference substance concentration (X) in the range of 2.3 to 23μg/ml (r = 0.9991). The average recovery of formononetin is 96.93% with the RSD of 1.30%. Conclusion: The method is convenient and accurate, and can be used for the quality determination of corn silk.

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