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当前位置: 首页 > 文章 > Saccharomyces cerevisiae B9发酵生产S-腺苷甲硫氨酸的优化研究 华中农业大学学报 2006,25 (3) 254-258
Position: Home > Articles > Optimization of Sadenosylethionine Production by Saccharomyces cerevisiae B9 Journal of Huazhong Agricultural University 2006,25 (3) 254-258

Saccharomyces cerevisiae B9发酵生产S-腺苷甲硫氨酸的优化研究

作  者：
薛学东;袁德军;林开春
单  位：
华中农业大学生命科学技术学院;华中农业大学植物科学技术学院
关键词：
S-腺苷甲硫氨酸;发酵;优化
摘  要：
以高产S-腺苷甲硫氨酸的菌株Saccharomyces cerevisiaeB9对培养基和发酵条件进行了优化研究。通过单因素水平测试和正交水平测试,确定了发酵培养基的最佳配方组合为:蔗糖8%、NH4Cl 0.5%、NH4H2PO40.2%、酵母提取物1.2%、MgSO4.7H2O 0.01%、KH2PO40.6%、L-甲硫氨酸1.2%,与原始发酵培养基相比,S-腺苷甲硫氨酸从1.16 g/L提高到3.35 g/L,菌体干重从16.18 g/L提高到18.25 g/L。在此基础上,对发酵条件进行优化,结果发现:初始pH5,培养温度30℃,摇床转速180 r/min为最佳培养条件。此时,菌体干重达19.86 g/L,S-腺苷甲硫氨酸产率达4.2 g/L。
译  名：
Optimization of Sadenosylethionine Production by Saccharomyces cerevisiae B9
作  者：
XUE Xuedong ~(1)) YUAN Dejun~(2)) LIN Kaichun~(1,3))(~(1))College of Plant Science and Technolgy,Huazhong Agricultural University,Wuhan 430070,China;~(2))College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;~(3))Wuhan Green Century Bioengineering Co.,Ltd.,Wuhan 430070,China)
关键词：
Sadenosylmethionine;fermentation;optimization
摘  要：
The optimum medium compositions and culture conditions were carried out on Saccharomyces cerevisiae B9 with high activity of Sadenosylmethionine.The optimum carbon source and nitrogen source were evaluated first.The concentrationsof inorganic salt,yeast extractedand the precursor inductor of L methionine were also determined.Afterwards,bythe orthogonal experiment,the optimization concentrations of fermentation medium compositions were determined as follows:sucrose 8%,NH_4Cl 0.5%,NH_4H_2PO_4 0.2%,yeast soaked 1.2%,MgSO_4·7H_2O 0.1%,KH_2PO_4 0.6%,Lmethionine 1.2%.Under the optimum medium,in contrast with primitive fermentation medium,the yield of Sadenosylmethionine increased from 1.16 g/L to 3.35 g/L,mycelium dry weight increased from 16.18(g/L) to 18.25 g/L.Experiments of the optimizationon other fermentation conditionswere also carried out based on the above,the result showed that:initial pH=5,temperature 30℃,table rotational speed 180 r/min,under the optimization condition,the yield of Sadenosylmethionine was as high as 4.2 g/L,mycelium dry weight was as high as 19.86 g/L.

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